Vignali D A, Bickle Q D, Taylor M G
Department of Medical Helminthology, Winches Farm Laboratories, London School of Hygiene and Tropical Medicine, St Albans, Herts.
Parasitology. 1988 Feb;96 ( Pt 1):49-61. doi: 10.1017/s0031182000081658.
Actively immunized mice, whole-body irradiated with 650 or 525 rad., manifested comparable levels of resistance to Schistosoma mansoni compared with unirradiated, immunized mice in spite of a marked reduction in circulating leucocytes (greater than 90%) and platelets (greater than 85%), and despite an abrogation of delayed-type hypersensitivity (DTH) (Type IV) response to schistosomular antigens (as determined by footpad swelling, 24 h after injection of antigen). However, limited histopathological comparison of lung sections from irradiated and unirradiated mice 7 days post-challenge showed that cellular reactions ('foci') around parasites were essentially similar in size and cellular composition except that in irradiated mice, eosinophils were poorly represented both in the foci and in lung tissue in general. Neither presumed immune complex-mediated (Type III, Arthus reaction) hypersensitivity (as determined by footpad swelling, 5 h after injection of antigen) nor serum anti-schistosomulum extract antibody levels (as determined by ELISA) were affected. In addition, the pattern of 125I-labelled schistosomular surface antigens immunoprecipitated with serum from irradiated and unirradiated mice was essentially similar. These results are consistent with antibody playing an important role in vaccine-induced immunity in mice but suggest that radiosensitive T cell function and radiosensitive cells, such as platelets and polymorphonuclear cells, including eosinophils, may not be essential.
用650拉德或525拉德进行全身照射的主动免疫小鼠，尽管循环白细胞（减少超过90%）和血小板（减少超过85%）显著减少，且对血吸虫抗原的迟发型超敏反应（IV型）（通过注射抗原24小时后足垫肿胀测定）消失，但与未照射的免疫小鼠相比，对曼氏血吸虫表现出相当的抵抗力水平。然而，对攻击后7天的照射和未照射小鼠的肺切片进行的有限组织病理学比较显示，寄生虫周围的细胞反应（“病灶”）在大小和细胞组成上基本相似，只是在照射小鼠中，病灶和整个肺组织中的嗜酸性粒细胞数量都很少。推测的免疫复合物介导的（III型，阿瑟反应）超敏反应（通过注射抗原5小时后足垫肿胀测定）和血清抗血吸虫提取物抗体水平（通过ELISA测定）均未受影响。此外，用照射和未照射小鼠的血清免疫沉淀的125I标记的血吸虫表面抗原模式基本相似。这些结果与抗体在小鼠疫苗诱导的免疫中起重要作用一致，但表明放射敏感的T细胞功能以及放射敏感细胞，如血小板和包括嗜酸性粒细胞在内的多形核细胞，可能并非必不可少。
