Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Hyderabad, Telangana, 500085, India.
Centre for Alternative Energy Options, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Hyderabad, Telangana, 500085, India.
Appl Biochem Biotechnol. 2020 Jan;190(1):30-43. doi: 10.1007/s12010-019-03087-x. Epub 2019 Jul 12.
Rapid development of the industrial and domestic sectors has led to the rise of several energy and environmental issues. In accordance with sustainable development and waste minimization issues, biohydrogen production along with biomethane production via two-stage fermentation process using microorganisms from renewable sources has received considerable attention. In the present study, biohythane production with simultaneous wastewater treatment was studied in a two-stage (Biohydrogen and Biomethane) fermentation process under anaerobic conditions. Optimization of high organic content (COD) distillery spent wash effluent (DSPW) with dilution using sewage wastewater was carried out. Addition of leachate as a nutrient source was also studied for effective biohythane production. The experimental results showed that the maximum biohythane production at optimized concentration (substrate concentration of 60 g/L with 30% of leachate as a nutrient source) was 67 mmol/L bio-H and with bio-CH production of 42 mmol/L. Graphical Abstract.
快速发展的工业和国内部门导致了一些能源和环境问题的出现。为了实现可持续发展和减少废物的目标，利用可再生资源中的微生物通过两阶段发酵工艺生产生物氢和生物甲烷已经引起了相当大的关注。在本研究中，采用厌氧条件下的两段式（生物氢和生物甲烷）发酵工艺进行了同时生产生物氢和处理废水的研究。对高有机物含量（COD）的酿酒厂废水（DSPW）进行了优化，采用污水进行稀释。还研究了添加渗滤液作为营养源来有效生产生物氢的方法。实验结果表明，在优化浓度（底物浓度为 60g/L，以 30%的渗滤液作为营养源）下，最大生物氢产量为 67mmol/L，生物-CH 产量为 42mmol/L。
