生物量依赖型环境 DNA 排放的研究：以日本鲐幼鱼为例。
Biomass-dependent emission of environmental DNA in jack mackerel Trachurus japonicus juveniles.
机构信息
Maizuru Fisheries Research Station, Field Science Education and Research Center, Kyoto University, Maizuru, Kyoto, Japan.
Department of Zoology, Graduate School of Science, Kyoto University, Kyoto, Japan.
出版信息
J Fish Biol. 2019 Sep;95(3):979-981. doi: 10.1111/jfb.14095. Epub 2019 Jul 31.
Environmental DNA (eDNA) from juvenile jack mackerel Trachurus japonicus was detected in tanks with 1, 3, 10, or 30 individuals per tank. Quantitative PCR using a set of species-specific primers and a probe revealed that the concentration of eDNA increased almost linearly with the density of fish. The coefficient of determination (R ) in the linear regression was lower than values previously reported for freshwater fishes in similar settings.
从每个水箱 1、3、10 或 30 条幼鱼中检测到了鲐鱼（Trachurus japonicus）的环境 DNA（eDNA）。使用一组物种特异性引物和探针的定量 PCR 显示，eDNA 的浓度几乎随鱼类密度呈线性增加。线性回归的决定系数（R）低于以前在类似环境中报道的淡水鱼类的值。
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