College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China.
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China.
Comp Immunol Microbiol Infect Dis. 2019 Aug;65:181-188. doi: 10.1016/j.cimid.2019.05.013. Epub 2019 May 18.
Goatpox (GTP), sheeppox (SPP) and lumpy skin disease (LSD) are three severe diseases of goat, sheep and cattle. Their typical clinical symptoms are characterized by vesicles, papules, nodules, pustules and scabs on animal skins. The GTP, SPP and LSD are caused by goatpox virus (GTPV), sheeppox virus (SPPV) and lumpy skin disease virus (LSDV), respectively, all of which belong to the genus Capripoxvirus in the family Poxviridae. Several capripoxvirus (CaPV) isolates have been virulently attenuated through serial passaging in vitro for production of live vaccines. CaPV-based vector systems have been broadly used to construct recombinant vaccines for delivering foreign antigens, many of which have been demonstrated to induce effective immune protections. Homologous recombination is the most commonly used method for constructing recombinant CaPVs. Here, we described a methodology for generation of recombinant CaPVs by the homologous recombination, and further reviewed CaPV-vectored vaccines for delivering foreign antigens.
山羊痘（GTP）、绵羊痘（SPP）和牛结节性皮肤病（LSD）是山羊、绵羊和牛的三种严重疾病。它们的典型临床症状是动物皮肤上出现水疱、丘疹、结节、脓疱和结痂。GTP、SPP 和 LSD 分别由山羊痘病毒（GTPV）、绵羊痘病毒（SPPV）和牛结节性皮肤病病毒（LSDV）引起，均属于痘病毒科山羊痘病毒属。通过在体外连续传代，已经使几种山羊痘病毒（CaPV）分离株毒力减弱，用于生产活疫苗。CaPV 载体系统已广泛用于构建用于传递外源抗原的重组疫苗，其中许多已被证明可诱导有效的免疫保护。同源重组是构建重组 CaPV 的最常用方法。在这里，我们描述了一种通过同源重组生成重组 CaPV 的方法，并进一步综述了用于传递外源抗原的 CaPV 载体疫苗。
