Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City 402, Taiwan.
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City 402, Taiwan.
Comp Immunol Microbiol Infect Dis. 2019 Aug;65:96-102. doi: 10.1016/j.cimid.2019.05.008. Epub 2019 May 9.
Porcine reproductive and respiratory syndrome virus (PRRSV) has been endemic in Taiwan since 1991. This study aimed to present a highly virulent PRRSV in Taiwan based on farm data collection and both in vitro and in vivo evaluations in virus challenge studies. This virulent PRRSV strain was first noticed on Farm TSYM due to continuously high nursery mortality rate and severe PRRSV-associated pneumonia. In phylogenetic surveillance, the PRRSV TSYM-strain remained in the predominant position for years, even with several other PRRSV strain invasions. In laboratory challenge trials, the TSYM-strain led to prolonged pyrexia, growth retardation, high mortality rates and high viremia titer that similar to the highly pathogenic PRRSV. The TSYM-strain isolate also triggered early interleukin-10 up-regulation and significantly higher infection rates under in vitro experiments. This study provides information of a comparably virulent strain in Taiwan and its appearance in both farm and laboratory levels.
猪繁殖与呼吸综合征病毒（PRRSV）自 1991 年以来在台湾流行。本研究旨在根据农场数据收集以及病毒攻毒研究中的体外和体内评估，介绍台湾一种高致病性的 PRRSV。这种高致病性的 PRRSV 株首先在 TSYM 农场被发现，因为其保育猪的死亡率持续居高不下，且与 PRRSV 相关的肺炎非常严重。在系统进化监测中，PRRSV TSYM 株多年来一直处于主要地位，即使有其他几种 PRRSV 株的入侵。在实验室攻毒试验中，TSYM 株导致发热时间延长、生长迟缓、高死亡率和高病毒血症滴度，与高致病性 PRRSV 相似。TSYM 株分离株在体外实验中还引发了早期白细胞介素-10 的上调，并显著提高了感染率。本研究提供了台湾一种相当毒力的菌株的信息及其在农场和实验室水平的表现。
