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Chemical modification of lysine residues at active-site of human placental estradiol 17 beta-dehydrogenase.

作者信息

Inano H

机构信息

National Institute of Radiological Sciences, Chiba-shi, Japan.

出版信息

Biochem Biophys Res Commun. 1988 Apr 29;152(2):789-93. doi: 10.1016/s0006-291x(88)80107-4.

DOI:10.1016/s0006-291x(88)80107-4
PMID:3130054
Abstract

Estradiol 17 beta-dehydrogenase (EC 1.1.1.62.) activity was decreased by 2,4,6-trinitrobenzene sulfonate (TNBS), a reagent for modification of epsilon-amino moiety of lysine residues in a protein. The inactivation exhibited pseudo-first-order kinetics, and was protected by oxidyzed cofactors. Stoichiometric studies showed that the complete inactivation was caused by modification of one lysine residue per molecule of the enzyme. Differential modification with 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB), TNBS and dithiothreitol (DTT) indicated that the residues of lysine and cysteine were located at the active-site and played an essential role in the catalytic function of the estradiol 17 beta-dehydrogenase.

摘要

相似文献

1
Chemical modification of lysine residues at active-site of human placental estradiol 17 beta-dehydrogenase.
Biochem Biophys Res Commun. 1988 Apr 29;152(2):789-93. doi: 10.1016/s0006-291x(88)80107-4.
2
Chemical modification of tyrosine residues in active-site of human placental estradiol 17 beta-dehydrogenase by tetranitromethane.用四硝基甲烷对人胎盘雌二醇17β-脱氢酶活性位点的酪氨酸残基进行化学修饰。
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Affinity labeling of the cofactor-binding site of estradiol 17 beta-dehydrogenase of human placenta by 5'-p-fluorosulfonylbenzoyl adenosine.
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Reactivation of human placental 17 beta,20 alpha-hydroxysteroid dehydrogenase affinity alkylated by estrone 3-(bromoacetate): topographic studies with 16 alpha-(bromoacetoxy)estradiol 3-(methyl ether).由雌酮3 -（溴乙酸酯）烷基化的人胎盘17β,20α - 羟基类固醇脱氢酶亲和力的重新激活：用16α -（溴乙酰氧基）雌二醇3 -（甲醚）进行的拓扑学研究
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Human placental 17 beta-estradiol dehydrogenase and 20 alpha-hydroxysteroid dehydrogenase. Two activities at a single enzyme active site.人胎盘17β-雌二醇脱氢酶和20α-羟基类固醇脱氢酶。单一酶活性位点上的两种活性。
J Biol Chem. 1981 Jan 10;256(1):316-21.

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