Department of Biology, Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand.
Department of Biochemistry, Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand.
Environ Sci Pollut Res Int. 2019 Sep;26(26):26765-26781. doi: 10.1007/s11356-019-05905-5. Epub 2019 Jul 12.
Microbial communities in groundwater are diverse and each may respond differently to environmental change. The goal of this study was to investigate the diversity, abundance, and dynamics of microbial communities in impacted groundwater and correlate them to the corresponding land use and groundwater geochemistry, using an Illumina MiSeq platform targeting the V3 and V4 regions of the 16S rRNA gene. The resulting MiSeq sequencing revealed the co-occurrence patterns of both abundant and rare microbial taxa within an impacted groundwater basin. Proteobacteria were the most common groundwater-associated bacterial phylum, mainly composed of the classes Gammaproteobacteria, Betaproteobacteria, Alphaproteobacteria, and Deltaproteobacteria. The phyla detected at less abundances were the Firmicutes, Bacteroidetes, Planctomycetes, Actinobacteria, OD1, and Nitrospirae. The members of detected groundwater microorganisms involved in natural biogeochemical processes such as nitrification, anammox, methane oxidation, sulfate reduction, and arsenic transformation. Some of the detected microorganisms were able to perform anaerobic degradation of organic pollutants. The resulting PCA indicates that major land usage within the sampling area seemed to be significantly linked to the groundwater microbial distributions. The distinct microbial pattern was observed in the groundwater collected from a landfill area. This study suggests that the combinations of anthropogenic and natural effects possibly led to a unique pattern of microbial diversity across different locations at the impacted groundwater basin.
地下水中的微生物群落多样，每个群落对环境变化的反应可能不同。本研究的目的是利用 Illumina MiSeq 平台，针对 16S rRNA 基因的 V3 和 V4 区域，调查受影响地下水中微生物群落的多样性、丰度和动态，并将其与相应的土地利用和地下水地球化学相关联。MiSeq 测序结果揭示了受影响地下水盆地中丰富和稀有微生物类群的共同发生模式。变形菌门是与地下水关联最密切的细菌门，主要由γ变形菌纲、β变形菌纲、α变形菌纲和δ变形菌纲组成。丰度较低的门是厚壁菌门、拟杆菌门、浮霉菌门、放线菌门、OD1 和硝化螺旋菌门。检测到的参与自然生物地球化学过程的地下水中微生物成员，如硝化作用、厌氧氨氧化、甲烷氧化、硫酸盐还原和砷转化。一些检测到的微生物能够进行有机污染物的厌氧降解。所得 PCA 表明，采样区域内的主要土地利用似乎与地下水微生物分布有显著关联。在垃圾填埋场地区采集的地下水观察到了明显的微生物模式。本研究表明，人为和自然因素的组合可能导致受影响地下水盆地不同地点的微生物多样性呈现独特模式。
