Department of Pure Chemistry, Faculty of Chemistry, Shahid Beheshti University G.C, Tehran, Iran.
Department of Pure Chemistry, Faculty of Chemistry, Shahid Beheshti University G.C, Tehran, Iran.
Steroids. 2019 Nov;151:108446. doi: 10.1016/j.steroids.2019.108446. Epub 2019 Jul 11.
In this study, the biotransformation of progesterone (1) and testosterone enanthate (5) using the whole cells of Circinella muscae was investigated for the first time. Microbial transformation of 1 with C. muscae afforded three known metabolites including 9α-hydroxyprogesterone (2), 14α-hydroxyprogesterone (3) and 6β,14α dihydroxyprogesterone (4) after 6 days of incubation at 26 °C. The biotransformation of 5 with C. muscae yielded a new metabolite; 8β,14α-dihydroxytestosterone (8), in addition to two known metabolites; 6β-hydroxytestosterone (6), and 9α-hydroxytestosterone (7). The structure of the metabolites were established on the basis of spectroscopic data.
本研究首次考察了 Circinella muscae 全细胞对孕酮（1）和庚酸睾酮（5）的生物转化。在 26°C 孵育 6 天后，微生物转化 1 得到三种已知代谢产物，包括 9α-羟基孕酮（2）、14α-羟基孕酮（3）和 6β,14α-二羟基孕酮（4）。用 C. muscae 对 5 进行生物转化除了得到两种已知代谢产物 6β-羟睾酮（6）和 9α-羟睾酮（7）外，还生成了一种新的代谢产物 8β,14α-二羟基睾酮（8）。根据光谱数据确定了代谢产物的结构。
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