Gilead L, Rahamim E, Ziv I, Or R, Razin E
Institute of Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Immunology. 1988 Apr;63(4):669-75.
Homogeneous populations of human mast cells were differentiated and grown by culturing bone marrow cells in the presence of conditioned medium derived from lectin-stimulated human peripheral blood mononuclear cells. The cells obtained were similar in ultrastructure, proteoglycan type and lipid products generated upon calcium ionophore A23187, and immunological activation to the murine E-mast cells (E-MC) differentiated in culture containing IL-3. Fluorescence analysis revealed that the human E-MC expressed IgE-Fc receptors which retained bound IgE through several washes. These cells did not express cell-surface lymphoid determinants (T11, T4, T8 and B4) and myeloid determinants 'My'. However, 40% of these cells expressed monocytic surface determinants, such as M-1. The amount of histamine that was found per 10(6) cells was 525 +/- 106 ng (mean +/- SE, n = 4). These cultured mast cells possessed granular chondroitin sulphate E proteoglycan of about 180,000 MW. Following activation with either calcium ionophore A23187 or anti-hIgE challenge, these mast cells released their preformed mediators and generated mainly leukotriene C4 leukotriene B4, and platelet-activating factor. In conclusion, according to all of these criteria, these human cultured mast cells show many similarities to the murine cultured E-mast cells, and therefore could be considered as the culture analogue of the human intestinal E-mast cells identified recently.
通过在源自凝集素刺激的人外周血单核细胞的条件培养基存在下培养骨髓细胞，分化并培养出人肥大细胞的同质群体。所获得的细胞在超微结构、蛋白聚糖类型以及钙离子载体A23187作用下产生的脂质产物方面相似，并且在免疫激活方面与在含IL-3的培养基中分化的鼠E-肥大细胞（E-MC）相似。荧光分析显示，人E-MC表达IgE-Fc受体，经多次洗涤后仍保留结合的IgE。这些细胞不表达细胞表面淋巴细胞决定簇（T11、T4、T8和B4）以及髓系决定簇“My”。然而，这些细胞中有40%表达单核细胞表面决定簇，如M-1。每10(6)个细胞中组胺的含量为525 +/- 106 ng（平均值 +/- 标准误，n = 4）。这些培养的肥大细胞具有约180,000 MW的颗粒状硫酸软骨素E蛋白聚糖。在用钙离子载体A23187或抗人IgE激发激活后，这些肥大细胞释放其预先形成的介质，主要产生白三烯C4、白三烯B4和血小板活化因子。总之，根据所有这些标准，这些人培养的肥大细胞与鼠培养的E-肥大细胞有许多相似之处，因此可被视为最近鉴定出的人肠道E-肥大细胞的培养类似物。
