Rafael Alves Santana Cesário Francisco, Rodrigues de Albuquerque Thaís, Mendes de Lacerda Giovana, Rayane Correia de Oliveira Maria, Anderson Fernandes da Silva Bruno, Bezerra Rodrigues Lindaiane, Oliveira Brito Pereira Bezerra Martins Anita, Roberto Guedes da Silva Almeida Jackson, Lima Vale Mariana, Douglas Melo Coutinho Henrique, Rose Alencar de Menezes Irwin
Department of Chemical Biology, Molecular Bioprospecting Postgraduate Program, Regional University of Cariri, 63105-000 Crato, CE, Brazil.
Natural Resources of the Semi-Arid Program, Federal University of the Vale São Francisco, Av. José de Sá Maniçoba, s/n, Campus Universitário, Petrolina, PE, Brazil.
Saudi J Biol Sci. 2019 Jul;26(5):873-880. doi: 10.1016/j.sjbs.2018.01.008. Epub 2018 Jan 31.
Inflammation is a protective response of the organism against damaging agents, this process is considered beneficial, however in some situations, this response can be damage when exacerbated effect are present. This claim objective to evaluate the qualitative and quantitative chemical profile, acute toxic and anti-inflammatory effects of the hydroalcoholic extract of leaves from (Cham. & Schlecht.) K. Schum (HELTF). Quantitative and qualitative phytochemical analysis was performed by HPLC-DAD and colorimetric assay. The topical anti-inflammatory activity was determined in Croton oil-induced ear edema assay and systemic activity was performed in vascular permeability, paw edema induced by carrageenan and dextran. Phytochemical analysis of leaves from HELTF showed presence of tannin, flavonoid, saponins an other that confirmed by HPLC analysis. The extract did not cause significant with LD greater than 5000 mg/kg and did not promote significate reduction in topical inflammatory process. However, HELTF demonstrate significant reduction of paw edema induced by carrageenan and dextran. The HELTF (200 mg/kg) reduced the protein/cell migration in the intradermal carrageenan-induced inflammation. Our results demonstrated that the first time the chemical profile and describe the effective action in systemic anti-inflammatory, antiedematogenic activity and low acute toxicity. This activity presents, supporting its traditional use. However, new studies are necessary for the detection and clarification of the possible mechanism of action.
炎症是机体对损伤因子的一种保护性反应，这一过程被认为是有益的，然而在某些情况下，当出现加剧效应时，这种反应可能会造成损害。本研究旨在评估（Cham. & Schlecht.）K. Schum的叶水醇提取物（HELTF）的定性和定量化学特征、急性毒性和抗炎作用。通过高效液相色谱-二极管阵列检测法（HPLC-DAD）和比色法进行定量和定性植物化学分析。在巴豆油诱导的耳肿胀试验中测定局部抗炎活性，在血管通透性试验、角叉菜胶和右旋糖酐诱导的爪肿胀试验中测定全身活性。对HELTF的叶进行植物化学分析表明存在单宁、黄酮类化合物、皂苷等，这通过HPLC分析得到证实。提取物在半数致死量大于5000 mg/kg时未引起显著影响，且未促进局部炎症过程的显著减轻。然而，HELTF显示对角叉菜胶和右旋糖酐诱导的爪肿胀有显著减轻作用。HELTF（200 mg/kg）减少了角叉菜胶诱导的皮内炎症中的蛋白质/细胞迁移。我们的结果首次证明了其化学特征，并描述了其在全身抗炎、抗水肿活性和低急性毒性方面的有效作用。这种活性支持了其传统用途。然而，需要进行新的研究来检测和阐明可能的作用机制。
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