Regional University of Cariri, Brazil.
Federal University of Paraíba, Brazil.
Complement Ther Med. 2019 Aug;45:254-261. doi: 10.1016/j.ctim.2019.06.001. Epub 2019 Jun 4.
The objective of the present study was to evaluate the systemic anti-inflammatory activity of the hydroalcoholic extract of the leaves of Licania rigida Benth (EHFLR) on models of systemic inflammation in mice.
The quantitative chemical profiles of phenolic acids and flavonoids were performed by High-Performance Liquid Chromatography (HPLC). Systemic anti-inflammatory activity was determined from carrageenan and dextran-induced paw edema models and the animals were orally treated (p.o.) with EHFLR at doses of 25, 50, 100 mg/kg, indomethacin (10 mg/kg) for carrageenan-induced paw edema and promethazine (6 mg/kg) for dextran-induced paw edema. The possible mechanisms involved in the anti-inflammatory action of the extract were evaluated by the paw edema models induced by histamine and arachidonic acid, and by the model of carrageenan-induced peritonitis, where vascular permeability and leukocyte migration to the peritoneal cavity were evaluated.
The results of the HPLC identified the presence of phenolic acids and flavonoids, with chlorogenic acid (1.16%) and Caempferol (0.81%) as the main constituents. From the results, it was concluded that the extract has an LD ≥5000 mg/kg when administered orally in mice as this dose did not trigger deaths in any of the observed groups. EHFLR (25 mg/kg) showed a significant antiderematogenic effect on histamine and arachidonic acid-induced paw edema at the third hour of the tests, with a percentage of inhibition of 46.64% and 18.33%, respectively. The extract (25 mg/kg, p.o.) also significantly reduced vascular permeability and leukocyte migration in the peritoneal cavity.
It is concluded that EHFLR exerts a systemic anti-inflammatory action, which seems to depend, at least in part, on the inhibition of arachidonic acid metabolism and the action of vasoactive amines. In addition, the extract reduced the leukocyte migration in the peritoneal cavity, indicating that its action may be linked to the inhibition of pro-inflammatory cytokines.
本研究旨在评估利坎尼亚·里吉达·本斯（Licania rigida Benth）叶的水醇提取物（EHFLR）在小鼠全身炎症模型中的系统抗炎活性。
采用高效液相色谱法（HPLC）对酚酸和类黄酮的定量化学特征进行分析。通过角叉菜胶和葡聚糖诱导的爪肿胀模型来确定系统抗炎活性，并通过口服给予 EHFLR（剂量为 25、50、100mg/kg）、吲哚美辛（10mg/kg，用于角叉菜胶诱导的爪肿胀）和苯海拉明（6mg/kg，用于葡聚糖诱导的爪肿胀）来治疗动物。通过组胺和花生四烯酸诱导的爪肿胀模型以及角叉菜胶诱导的腹膜炎模型评估提取物抗炎作用的可能机制，评估血管通透性和白细胞向腹腔的迁移。
HPLC 结果鉴定出存在酚酸和类黄酮，其中绿原酸（1.16%）和山奈酚（0.81%）为主要成分。结果表明，该提取物经口给予小鼠的 LD≥5000mg/kg 时不会引起任何观察到的组死亡。EHFLR（25mg/kg）在测试的第三小时对角叉菜胶和花生四烯酸诱导的爪肿胀显示出显著的抗致炎作用，抑制率分别为 46.64%和 18.33%。该提取物（25mg/kg，口服）还显著降低了腹腔内血管通透性和白细胞迁移。
EHFLR 发挥全身抗炎作用，这似乎至少部分依赖于抑制花生四烯酸代谢和血管活性胺的作用。此外，提取物减少了腹腔内白细胞的迁移，表明其作用可能与抑制促炎细胞因子有关。
