Luan Yunqi, Luan Yunpeng, Zhao Youjie, Xiong Fei, Li Yanmei, Liu Lili, Cao Yong, Dai Fei
Soil System, Department of Geography and Ecology Lomonosov Moscow State University, Russia.
Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, 650224, PR China.
Saudi J Biol Sci. 2019 Jul;26(5):1011-1022. doi: 10.1016/j.sjbs.2019.04.002. Epub 2019 Apr 5.
The roots of Forest ex diels (Fed), locally known as Tsoong, have been used as a tonic food. Tsoong has wide range of pharmacological effects, including anticancer efficacy. In the present study, the anticancer activity of Tsoong and its potential molecular mechanisms were investigated. Isorhamnetin, a flavonol aglycone, is important compound and metabolite in Tsoong. It can promote apoptosis of colon cancer cells through up-regulating apoptosis-related genes (Apaf1, Casp3 and Casp9) because it blocks Hsp70 genes (Hspa1a, Hspa1b and Hspa8). These were verified by and experiments. , cell counting kit-8 (CCK-8) assays and flow cytometry in HCT116 and SW480 colon cancer cell were used to assess the anti-proliferation and apoptosis-promoting activities of Tsoong. , the antitumor effect of Tsoong was assessed in colon cancer-bearing nude mice as a xenograft model. These results show that Isorhamnetin is very critical in Tsoong because Tsoong can down-regulate Hsp70 genes and promote apoptosis of colon cancer cells by inhibiting Hsp70 largely due to the efficacy of Isorhamnetin. Our results may ultimately help in the development of diagnostic and therapeutic strategies to control this devastating disease.
紫茎泽兰（学名：Eupatorium adenophorum Spreng.），当地称为“飞机草”，其根被用作滋补食品。飞机草具有广泛的药理作用，包括抗癌功效。在本研究中，对飞机草的抗癌活性及其潜在的分子机制进行了研究。异鼠李素，一种黄酮醇苷元，是飞机草中的重要化合物和代谢产物。它可以通过上调凋亡相关基因（Apaf1、Casp3和Casp9）来促进结肠癌细胞的凋亡，因为它阻断了热休克蛋白70基因（Hspa1a、Hspa1b和Hspa8）。这些通过……和……实验得到了验证。……，采用细胞计数试剂盒-8（CCK-8）检测法和流式细胞术对HCT116和SW480结肠癌细胞进行检测，以评估飞机草的抗增殖和促凋亡活性。……，以荷结肠癌裸鼠为异种移植模型评估飞机草的抗肿瘤作用。这些结果表明，异鼠李素在飞机草中非常关键，因为飞机草可以下调热休克蛋白70基因，并主要通过异鼠李素的功效抑制热休克蛋白70来促进结肠癌细胞的凋亡。我们的研究结果最终可能有助于制定控制这种毁灭性疾病的诊断和治疗策略。
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