Key Laboratory of Forestry Genetics & Biotechnology, Nanjing Forestry University, Ministry of Education, Nanjing, 210037, People's Republic of China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, No. 159 Longpan Road, Nanjing, 201137, People's Republic of China.
Appl Biochem Biotechnol. 2020 Jan;190(1):129-137. doi: 10.1007/s12010-019-03088-w. Epub 2019 Jul 15.
Apple pomace, an abundant accessible source of carbohydrate platform chemicals, is refractory to cellulase degradation because of the main barrier problem of pectin constitute. A rapid and portable method for the coproduction of pectin and fermentable sugars was developed using the pretreatment of acetic acid, followed by enzymatic hydrolysis. Compared with pectinase, acetic acid pretreatment provided the highest pectin yield of 19.1% and the highest enzymatic hydrolysis yield from apple pomace. The acidic pretreated apple pomace cellulose was easily and completely hydrolyzed into fermentable sugars. More than 98.2% conversion of cellulose was achieved in a batch hydrolysis using a cellulase loading of 25 FPU/g cellulose and 10% total solids without any special strategies. A mass balance analysis showed that 95.5 g pectin and 110.2 g fermentable sugars were produced from 500-g oven-dried apple pomace. The integrated process is suggestive of environment-friendly and recyclable methods for the industrial utilization of apple pomace.
苹果渣是一种丰富易得的碳水化合物平台化学品来源，但由于果胶构成的主要障碍问题，它难以被纤维素酶降解。本研究采用乙酸预处理，然后进行酶水解，开发了一种快速便携的果胶和可发酵糖联产方法。与果胶酶相比，乙酸预处理可获得 19.1%的最高果胶得率和来自苹果渣的最高酶水解得率。酸性预处理的苹果渣纤维素很容易且完全水解成可发酵糖。在纤维素酶用量为 25 FPU/g 纤维素和 10%总固体的分批水解中，无需任何特殊策略即可实现 98.2%以上的纤维素转化率。质量平衡分析表明，从 500 g 烘干的苹果渣中可生产 95.5 g 果胶和 110.2 g 可发酵糖。该综合工艺为苹果渣的工业利用提供了环保且可回收的方法。
