Ren Cong, Gong Wei, Li Feng, Xie Ming
Department of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, Liaoning, China.
Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, China.
Cell Mol Biol (Noisy-le-grand). 2019 Jun 30;65(5):24-31.
This study was carried out to evaluate the preventive and curative effects of Pilose antler against osteoporosis due to kidney deficiency, and investigate its potential mechanism of action. A model of osteoporosis due to kidney deficiency was established in rats using bilateral ovariectomy. Pilose antler polypeptide (PAP), Pilose antler polysaccharide (PAP'), and their mixture (PAP+PAP') were separately administered to the rats for 12 weeks, with progynova and xianlingubao tablets (XLGB) as the positive control groups. We determined the bone mineral density (BMD) and uterus Index of the rats. Osteoblastic bone metabolism-related indices in serum and bone tissue were measured with ELISA. Western blotting and RT-PCR were used to investigate the protein and mRNA expressions of Bmp-2, Smad1, Smad5, Runx2 in bone tissue. The morphology of bone tissue was determined using immunohistochemical methods. Compared with control group, PAP, PAP', PAP+PAP' increased BMD and regulated bone metabolism indices in serum and bone tissue. Treatment with Pilose antler up-regulated the mNRA and protein expressions of Bmp-2, Smad1, Smad5 and Runx2. Immunohistochemical staining showed that Bmp-2, Smad1, Smad5 and Runx2 were stained brown, indicating that all of them were positive. There were abnormal changes in the protein expressions of Bmp-2, Smad1, Smad5 and Runx2 in bone tissue, which may be an important mechanism underlying the development of kidney deficiency osteoporosis. Moreover, PAP, PAP' and PAP+PAP' had some preventive effects on osteoporosis, probably via the activation of the Bmp-2/Smad1 and Smad5/Runx2 signaling pathways through induction of high expressions of their mRNAs and proteins.
本研究旨在评价鹿茸对肾虚型骨质疏松症的防治作用，并探讨其潜在作用机制。采用双侧卵巢切除术建立大鼠肾虚型骨质疏松症模型。将鹿茸多肽（PAP）、鹿茸多糖（PAP'）及其混合物（PAP+PAP'）分别给予大鼠12周，以补佳乐和仙灵骨葆片（XLGB）作为阳性对照组。测定大鼠骨密度（BMD）和子宫指数。采用ELISA法检测血清和骨组织中成骨细胞骨代谢相关指标。采用蛋白质免疫印迹法和RT-PCR法检测骨组织中Bmp-2、Smad1、Smad5、Runx2的蛋白和mRNA表达。采用免疫组织化学方法观察骨组织形态。与对照组相比，PAP、PAP'、PAP+PAP'提高了大鼠BMD，调节了血清和骨组织中的骨代谢指标。鹿茸治疗上调了骨组织中Bmp-2、Smad1、Smad5和Runx2的mRNA和蛋白表达。免疫组织化学染色显示，Bmp-2、Smad1、Smad5和Runx2均呈棕色染色，表明均为阳性。骨组织中Bmp-2、Smad1、Smad5和Runx2蛋白表达存在异常变化，这可能是肾虚型骨质疏松症发病的重要机制。此外，PAP、PAP'和PAP+PAP'对骨质疏松症有一定的预防作用，可能是通过诱导其mRNA和蛋白的高表达激活Bmp-2/Smad1和Smad5/Runx2信号通路实现的。
