Caglar Hifa Gulru, Selek Sahabettin, Koktasoglu Fatmanur, Koyuncu Ismail, Demirel Metin, Sarikaya Alime, Meydan Sedat
Department of Medical Biochemistry, Faculty of Medicine, Bezmialem Vakif University, Adnan Menderes Bulvarı 34093, Istanbul, Turkey.
Department of Medical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa-Mardin Karayolu Uzeri 18.Km, Sanliurfa, Turkey.
Cell Mol Biol (Noisy-le-grand). 2019 Jun 30;65(5):79-86.
This study is aimed to investigate the effects of Camellia sinensis (CS), Hypericum perforatum (HP) and Urtica dioica (UD) in kidney and liver injury induced by carbon tetrachloride (CCl4) in rats. Highly toxic CCl4 which is used as a solvent in industry comprises experimental toxicity in rats and is widely used in hepatotoxicity and other tissue injury models. The purpose of this investigation is to monitor blood and various tissues by biochemical and histopathological analysis for preventive effects of CS, HP and UD on oxidative stress induced by administration of CCl4 and to enlighten the probable mechanism. Fifty eight rats were divided into five groups; sham group (Group 1, untreated animals), control CCl4 treated group (Group 2), HP extract-treated group (Group 3), UD extract-treated group (Group 4), CS extract-treated group (Group 5). All rats were anaesthetized at the end of the experiment and the blood was collected from each rat. Afterwards, tissue specimens were obtained. The tissue specimens were immersed in 10% formaldehyde for 24 hours. After routine tissue processing, the liver, kidney and stomach were sectioned in 5µm thickness, stained in hematoxylin and eosin. The histological study was performed by using light microscope. The serum marker enzymes were found to be significantly increased in CCl4-induced liver and kidney damage when compared with the sham group (p<0.05). However, treatment with CS, HP, and UD extracts resulted in decreased activity of serum enzymes. Malondialdehyde (MDA) levels were decreased by 20.51±0.95, 27.98±1.58, and 32.39±3.1 nmol/g wet weight protein in kidney homogenates and 16.65±1.75, 17.22±0.71 and 18.92±71 nmol/g wet weight protein in liver homogenates in CS, HP and UD treated groups, respectively. Our results have shown that additive antioxidants like CS, HP and UD will aid in diminishing these deviations in cases of liver and kidney dysfunction.
本研究旨在探讨茶树（CS）、贯叶连翘（HP）和异株荨麻（UD）对四氯化碳（CCl4）诱导的大鼠肝肾损伤的影响。高毒性的CCl4在工业上用作溶剂，对大鼠具有实验毒性，广泛应用于肝毒性和其他组织损伤模型。本研究的目的是通过生化和组织病理学分析监测血液和各种组织，以观察CS、HP和UD对CCl4给药诱导的氧化应激的预防作用，并阐明可能的机制。58只大鼠分为五组：假手术组（第1组，未处理动物）、CCl4处理对照组（第2组）、HP提取物处理组（第3组）、UD提取物处理组（第4组）、CS提取物处理组（第5组）。实验结束时，所有大鼠均麻醉，采集每只大鼠的血液。随后，获取组织标本。将组织标本浸入10%甲醛中24小时。经过常规组织处理后，将肝脏、肾脏和胃切成5μm厚的切片，用苏木精和伊红染色。使用光学显微镜进行组织学研究。与假手术组相比，CCl4诱导的肝损伤和肾损伤中血清标志物酶显著升高（p<0.05）。然而，CS、HP和UD提取物治疗导致血清酶活性降低。CS、HP和UD处理组的肾匀浆中丙二醛（MDA）水平分别降低了20.51±0.95、27.98±1.58和32.39±3.1 nmol/g湿重蛋白，肝匀浆中分别降低了16.65±1.75、17.22±0.71和18.92±71 nmol/g湿重蛋白。我们的结果表明，CS、HP和UD等添加型抗氧化剂有助于减轻肝肾功能障碍情况下的这些偏差。
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