Oguz Serhat, Kanter Mehmet, Erboga Mustafa, Toydemir Toygar, Sayhan Mustafa Burak, Onur Hatice
Department of General Surgery, Faculty of Medicine, Trakya University, Edirne, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Istanbul Medeniyet University, Istanbul, Turkey
Toxicol Ind Health. 2015 May;31(5):475-84. doi: 10.1177/0748233713480211. Epub 2013 Mar 1.
The present study was performed to investigate the effect of Urtica dioica (UD) on liver regeneration after partial hepatectomy (PH) in rats. A total of 24 male Sprague Dawley rats were divided into three groups: sham-operated, PH and PH + UD; each group contains eight animals. The rats in UD-treated groups were given UD oils (2 ml/kg/day) once a day orally for 7 days starting 3 days prior to hepatectomy operation. At day 7 after resection, liver samples were collected. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) were estimated in liver homogenates. Moreover, histopathological examination, mitotic index (MI), proliferating cell nuclear antigen labeling, proliferation index (PI), transferase-mediated deoxyuridine triphosphate nick end-labeling assay, apoptotic index (AI) were evaluated at day 7 after hepatectomy. As a result, UD significantly increased MI and PI, significantly decreased AI and also attenuated hepatic vacuolar degeneration and sinusoidal congestion in PH rats. UD treatment significantly decreased the elevated tissue MDA level and increased the reduced SOD activity and GSH level in the tissues. These results suggest that UD pretreatment was beneficial for rat liver regeneration after partial hepatectomy.
本研究旨在探讨荨麻对大鼠部分肝切除术后肝脏再生的影响。将24只雄性Sprague Dawley大鼠分为三组:假手术组、部分肝切除组和部分肝切除+荨麻组;每组包含8只动物。荨麻治疗组的大鼠在肝切除术前3天开始每天口服一次荨麻油(2毫升/千克/天),持续7天。切除术后第7天,采集肝脏样本。测定肝匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽(GSH)的水平。此外,在肝切除术后第7天评估组织病理学检查、有丝分裂指数(MI)、增殖细胞核抗原标记、增殖指数(PI)、转移酶介导的脱氧尿苷三磷酸缺口末端标记法、凋亡指数(AI)。结果显示,荨麻显著增加了MI和PI,显著降低了AI,还减轻了部分肝切除大鼠的肝空泡变性和窦状充血。荨麻治疗显著降低了升高的组织MDA水平,并提高了组织中降低的SOD活性和GSH水平。这些结果表明,荨麻预处理对大鼠部分肝切除术后的肝脏再生有益。