Wood M R, Wagner R C, Andrews S B, Greener D A, Williams S K
School of Life and Health Sciences, University of Delaware, Newark 19716.
Microcirc Endothelium Lymphatics. 1986;3(5-6):323-58.
Cultured, human endothelial cells from the iliac vein were divided into two groups for rapid-freezing using liquid helium in a Heuser-type cryopress freezing machine. The first group of cells was prefixed in buffered 2% glutaraldehyde solution whereas the second group was freshly-frozen. Following freeze substitution and embedding, morphometric analysis was carried out on thin sections of groups of endothelial cells. Measurements showed that glutaraldehyde prefixation significantly increases the numerical and volume densities of the vesicles compared to those of the freshly-frozen cells. However, the fact that no significant increase was demonstrated either in the numerical or volume densities of the caveolae, and that no significant change was observed in the ratio of the cell perimeter to area, suggests that the membrane source for these extra vesicles is internally generated. Studies are currently under way to identify the source of this membrane.
取自髂静脉的培养人内皮细胞被分成两组，使用休泽尔型冷冻机中的液氮进行快速冷冻。第一组细胞用2%戊二醛缓冲溶液固定，而第二组细胞则直接冷冻。经过冷冻置换和包埋后，对内皮细胞组的薄片进行形态计量分析。测量结果表明，与直接冷冻的细胞相比，戊二醛固定显著增加了小泡的数量密度和体积密度。然而，小窝的数量密度或体积密度没有显著增加，并且细胞周长与面积的比率也没有显著变化，这表明这些额外小泡的膜来源是内部产生的。目前正在进行研究以确定这种膜的来源。
