The People's Hospital of Shapingba District in Chongqing, Chongqing, China.
Institute of Infectious Diseases, Southwest Hospital, Army Medical University, Chongqing, China.
Cancer Biomark. 2019;26(1):31-39. doi: 10.3233/CBM-182257.
Hepatocellular carcinoma (HCC) is a common cancer and exhibits high morbidity and mortality in the world. We recently identified LHX3 as a preferentially expressed gene with a possible involvement in HCC.
To determine the expression, clinical relevance, prognostic significance and functions of LHX3 in HCC.
LHX3 expression was assessed in 190 cancerous and 40 adjacent non-cancerous tissues by PCR, western blot and immunohistochemistry. Associations between LHX3 expression and clinicopathological characteristics of patients were investigated. Correlations between LHX3 expression and overall survival of patients were analyzed by Kaplan-Meier and Cox-regression methods. Functional roles of LHX3 were evaluated by transwell assays.
LHX3 expression is significantly increased in carcinoma tissues, and associated with clinical stage and metastasis of patients. LHX3 expression is much higher in the advanced-stage patients than the early-stage patients, and is sharply increased in metastasic patients. High LHX3 expression is associated with unfavorable overall survival, and is an independent prognostic factor of patients. Moreover, LHX3 is an unfavorable and independent prognostic factor unique to advanced-stage patients. Knockdown expression of LHX3 obviously inhibits tumor cell migration and invasion.
LHX3 is an advanced-stage prognostic biomarker, and acts as a new potential metastatic oncogene in HCC.
肝细胞癌（HCC）是一种常见的癌症，在世界范围内具有较高的发病率和死亡率。我们最近发现 LHX3 是一种优先表达的基因，可能参与 HCC 的发生。
确定 LHX3 在 HCC 中的表达、临床相关性、预后意义和功能。
通过 PCR、western blot 和免疫组织化学检测 190 例癌组织和 40 例癌旁正常组织中 LHX3 的表达。分析 LHX3 表达与患者临床病理特征的关系。通过 Kaplan-Meier 和 Cox 回归方法分析 LHX3 表达与患者总生存率的相关性。通过 Transwell 测定评估 LHX3 的功能作用。
LHX3 的表达在癌组织中明显增加，与患者的临床分期和转移有关。晚期患者的 LHX3 表达明显高于早期患者，转移患者的 LHX3 表达明显升高。高 LHX3 表达与不良总生存率相关，是患者的独立预后因素。此外，LHX3 是晚期患者独特的不良和独立的预后因素。LHX3 的敲低表达明显抑制肿瘤细胞的迁移和侵袭。
LHX3 是晚期的预后生物标志物，是 HCC 中一种新的潜在的转移性癌基因。
