Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Anhui 233004, PR China; Anhui Key Laboratory of Tissue Transplantation, The First Affiliated Hospital of Bengbu Medical College, Anhui 233004, PR China.
Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, Anhui 233004, PR China; Department of Immunology, Bengbu Medical College, Anhui 233030, PR China.
Genomics. 2019 Jul;111(4):986-996. doi: 10.1016/j.ygeno.2018.06.006. Epub 2018 Jul 6.
The underlying mechanisms of macrophage polarization have been detected by genome-wide transcriptome analysis in a variety of mammals. However, the transcriptome profile of rat genes in bone marrow-derived macrophages (BMM) at different activation statuses has not been reported. Therefore, we performed RNA-Sequencing to identify gene expression signatures of rat BMM polarized in vitro with different stimuli. The differentially expressed genes (DEGs) among unactivated (M0), classically activated pro-inflammatory (M1), and alternatively activated anti-inflammatory macrophages (M2) were analyzed by using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis. In this study, not only we have identified the changes of global gene expression in rat M0, M1 and M2, but we have also made clear systematically the key genes and signaling pathways in the differentiation process of M0 to M1 and M2. These will provide a foundation for future researches of macrophage polarization.
通过对多种哺乳动物的全基因组转录组分析，已经发现了巨噬细胞极化的潜在机制。然而，不同激活状态下骨髓来源巨噬细胞（BMM）的大鼠基因转录组图谱尚未报道。因此，我们进行了 RNA 测序，以鉴定用不同刺激体外极化的大鼠 BMM 的基因表达特征。通过基因本体论和京都基因与基因组百科全书富集分析，分析未激活（M0）、经典激活促炎（M1）和替代激活抗炎巨噬细胞（M2）之间的差异表达基因（DEGs）。在这项研究中，我们不仅确定了大鼠 M0、M1 和 M2 中全局基因表达的变化，而且还系统地阐明了 M0 向 M1 和 M2 分化过程中的关键基因和信号通路。这些将为巨噬细胞极化的未来研究提供基础。
