Sod-Moriah U A, Sror U, Shemi D, Potashnik G, Chayoth R, Shaked I, Kaplanski J
Department of Biology, Ben Gurion University of the Negev, Beer Sheva/Israel.
Andrologia. 1988 Jan-Feb;20(1):60-6. doi: 10.1111/j.1439-0272.1988.tb02366.x.
Adult male rats were injected s.c. once a week for 3 weeks with DBCP, 20 mg/kg B.W. Animals were sacrificed 5, 9, 13, 17, 25 and 50 weeks after last injection. Body weight was recorded once a week. Prior to sacrifice each male was presented with proestral females in order to determine the male's mating behaviour and fertility. Testes were removed, weighed and taken for standard histological examination. DBCP treatment caused a reduction of body weight which reverted back to control levels some 17 weeks post injection. Testes weights were reduced and remained low despite the recovery of body weight. Generally, all males showed normal mating behaviour but most of them were infertile. Testicular histology showed a correlation between decreasing testicular weight and increasing percentage of degenerated seminiferous tubules, which was on the other hand correlated with decreasing tubular diameter. Serum levels of FSH and LH were significantly increased in the infertile DBCP treated males while values for the fertile ones were similar to those of controls. There were no differences in serum testosterone levels between DBCP treated and control animals. It is concluded that in DBCP treated rats testicular degenerative damages are associated with increased circulating gonadotrophin levels and with normal testosterone levels. Although mating behaviour is unaffected fertility is depressed and does not recover for at least 50 weeks post injection. It is suggested that DBCP treatment affects mainly the activity of the Sertoli cells while the Leydig cells are affected to a much lesser degree.
成年雄性大鼠每周皮下注射一次DBCP（20毫克/千克体重），持续3周。在最后一次注射后的5、9、13、17、25和50周处死动物。每周记录一次体重。在处死前，让每只雄性与处于动情前期的雌性接触，以确定雄性的交配行为和生育能力。取出睾丸，称重并进行标准组织学检查。DBCP处理导致体重下降，在注射后约17周恢复到对照水平。尽管体重恢复，但睾丸重量仍降低并维持在低水平。一般来说，所有雄性都表现出正常的交配行为，但大多数不育。睾丸组织学显示睾丸重量下降与退化的生精小管百分比增加之间存在相关性，而这又与管径减小相关。不育的DBCP处理雄性大鼠血清FSH和LH水平显著升高，而可育大鼠的值与对照相似。DBCP处理组和对照组动物的血清睾酮水平没有差异。结论是，在DBCP处理的大鼠中，睾丸退行性损伤与循环促性腺激素水平升高和正常睾酮水平有关。尽管交配行为未受影响，但生育能力下降，且在注射后至少50周未恢复。提示DBCP处理主要影响支持细胞的活性，而对睾丸间质细胞的影响程度要小得多。
