Jonchhe Sagun, Mao Hanbin
Department of Chemistry and Biochemistry, Kent State University, Kent, OH, USA.
Methods Mol Biol. 2019;2027:171-180. doi: 10.1007/978-1-4939-9616-2_14.
Single-molecule techniques such as fluorescence-based methods offer superior sensitivity in biosensing. By direct coupling of analyte recognition and signal amplification, a new sensing strategy, single-molecule mechanochemical sensing, has demonstrated high signal-to-noise ratio in the detection of chemicals and biochemicals. However, parallel sensing is limited. In this strategy, DNA origami nanoassemblies with different sensing probes are used as templates for multiplexing tasks. Assisted by the mechanochemical reporting units, these templates are capable of simultaneous detection of biological samples such as platelet-derived growth factor (PDGF) and DNA fragments in microfluidic channels. The origami-based strategy therefore increases multitasking compatibility of the mechanochemical sensing.
诸如基于荧光的方法等单分子技术在生物传感中具有卓越的灵敏度。通过将分析物识别与信号放大直接耦合，一种新的传感策略——单分子机械化学传感，已在化学物质和生物化学物质的检测中展现出高信噪比。然而，并行传感存在局限性。在该策略中，带有不同传感探针的DNA折纸纳米组件被用作多重任务的模板。在机械化学报告单元的辅助下，这些模板能够在微流控通道中同时检测生物样本，如血小板衍生生长因子（PDGF）和DNA片段。因此，基于折纸的策略提高了机械化学传感的多任务兼容性。
