Pearson Melanie M
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.
Methods Mol Biol. 2019;2021:5-13. doi: 10.1007/978-1-4939-9601-8_2.
Proteus mirabilis is generally easy to culture, but its tendency to swarm on a wide variety of media can interfere with isolation of single colonies or identification of other species in a sample. Therefore, specialized media may be needed to control swarming or to study the bacteria under chemically defined conditions. Here, methods are described for routine culture of P. mirabilis, isolation of P. mirabilis from mixed cultures, and culture of P. mirabilis on physiologically relevant media.
奇异变形杆菌通常易于培养,但其在多种培养基上蔓延生长的倾向可能会干扰单个菌落的分离或样本中其他菌种的鉴定。因此,可能需要使用特殊培养基来控制蔓延生长或在化学限定条件下研究该细菌。本文介绍了奇异变形杆菌的常规培养方法、从混合培养物中分离奇异变形杆菌的方法以及在生理相关培养基上培养奇异变形杆菌的方法。