免疫组织化学在揭示银屑病表皮角质形成细胞中 S100A7/JAB1 共定位中的作用。

Contribution of Immunohistochemistry in Revealing S100A7/JAB1 Colocalization in Psoriatic Epidermal Keratinocyte.

机构信息

Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.

出版信息

Methods Mol Biol. 2020;2109:67-74. doi: 10.1007/7651_2019_251.

DOI:10.1007/7651_2019_251

Abstract

The application of immunohistological methods provides an invaluable contribution in revealing the protein colocalization, which may reflect the occurrence of molecular interaction processes.This chapter describes comprehensive protocols for detection of S100A7/JAB1 colocalization by immunohistochemistry in archival formalin-fixed and paraffin-embedded skin biopsies from healthy and psoriatic subjects. In addition, we provide a protocol for immunocytochemical detection of S100A7/JAB1 colocalization in S100A7 CRISPR-activated human keratinocyte cell line.

摘要

免疫组织化学方法的应用在揭示蛋白质共定位方面提供了非常有价值的贡献，这可能反映了分子相互作用过程的发生。本章描述了通过免疫组织化学检测来自健康和银屑病受试者的福尔马林固定和石蜡包埋皮肤活检中 S100A7/JAB1 共定位的综合方案。此外，我们还提供了一种在 S100A7 CRISPR 激活的人角质形成细胞系中检测 S100A7/JAB1 共定位的免疫细胞化学检测方案。