Thoman M L, Weigle W O
Department of Immunology IMM9, Research Institute of Scripps Clinic, La Jolla, California 92037.
Cell Immunol. 1988 Jun;114(1):1-11. doi: 10.1016/0008-8749(88)90250-x.
The activation by concanavalin A (Con A) of murine lymphocytes derived from aged animals is impaired, as demonstrated by lower IL-2 synthesis, display of fewer IL-2 receptors, and less [3H]thymidine incorporation relative to similarly treated cells from young animals. The ability of the phorbol ester, phorbol 12-myristate 13-acetate (PMA), and the calcium ionophore, ionomycin, in conjunction with Con A, to improve these parameters of activation has been assessed. When splenic lymphocytes derived from aged mice are cultured with Con A plus ionomycin, IL-2 synthesis and IL-2 receptor expression are increased over the values obtained in parallel cultures triggered by Con A alone up to levels equal to that obtained in Con A-activated young cultures. The proliferative response is less sensitive to the augmenting effect of ionomycin. PMA is much less effective in improving these parameters of Con A activation. PMA plus ionomycin, in the absence of Con A, triggers cell cycle transition of lymphocytes derived from both aged and young animals. Both IL-2 synthesis and IL-2 receptor expression induced by PMA plus ionomycin reach levels equal or near equal that found in parallel cultures of cells from young animals; however, proliferation is lower than in young adult cultures.
与来自年轻动物的经类似处理的细胞相比，伴刀豆球蛋白A（Con A）对老龄动物来源的小鼠淋巴细胞的激活作用受损，表现为白细胞介素-2（IL-2）合成减少、IL-2受体表达较少以及[3H]胸苷掺入量减少。已评估佛波酯、佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）和钙离子载体离子霉素与Con A联合使用改善这些激活参数的能力。当用Con A加离子霉素培养老龄小鼠的脾淋巴细胞时，IL-2合成和IL-2受体表达相对于单独用Con A触发的平行培养物中的值有所增加，达到与Con A激活的年轻培养物中获得的值相当的水平。增殖反应对离子霉素的增强作用不太敏感。PMA在改善Con A激活的这些参数方面效果要差得多。在没有Con A的情况下，PMA加离子霉素可触发老龄和年轻动物来源的淋巴细胞的细胞周期转变。PMA加离子霉素诱导的IL-2合成和IL-2受体表达达到或接近与年轻动物细胞平行培养物中发现的水平；然而，增殖低于年轻成年培养物。
