Laboratory for Human Neurophysiology, Genetics and Stem Cells; South Australian Health and Medical Research Institute (SAHMRI), Adelaide, SA, Australia.
Laboratory for Human Neurophysiology, Genetics and Stem Cells; South Australian Health and Medical Research Institute (SAHMRI), Adelaide, SA, Australia; College of Medicine and Public Health, Flinders University, Adelaide, SA, Australia.
J Neurosci Methods. 2019 Sep 1;325:108350. doi: 10.1016/j.jneumeth.2019.108350. Epub 2019 Jul 13.
Advances in human cell reprogramming and induced pluripotent stem cell technologies generate tremendous potential for neuroscience studies in health and disease, while the neuroscientist toolbox for engineering a range of brain tissues and neuronal cell types is rapidly expanding. Here, we discuss how the emergence of new single-cell genomics methods may help benchmarking and optimizing the tissue engineering process. The inherent heterogeneity and variability of reprogrammed brain tissue may conceal important disease mechanisms if not accounted for by rigorous experimental design. Single-cell genomics methods may address this technical challenge and ultimately improve the development of new therapeutics for neurological and psychiatric disorders.
人类细胞重编程和诱导多能干细胞技术的进步为健康和疾病中的神经科学研究带来了巨大的潜力，而用于工程一系列脑组织和神经元细胞类型的神经科学家工具包也在迅速扩展。在这里，我们讨论了新的单细胞基因组学方法的出现如何帮助基准测试和优化组织工程过程。如果不以严格的实验设计来考虑，重编程脑组织的固有异质性和可变性可能会掩盖重要的疾病机制。单细胞基因组学方法可能会解决这一技术挑战，并最终改善神经和精神疾病新疗法的开发。
