Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Dec 5;223:117345. doi: 10.1016/j.saa.2019.117345. Epub 2019 Jul 9.
A novel luminescence energy transfer (LET) probe for detection of tumor related microRNAs using NaGdF:Yb,Er@NaYF upconversion nanoparticles (UCNPs) as energy donors and gold nanoparticles (AuNPs) as energy acceptors was developed. Using the double modified complementary DNA sequences of microRNA155 (miRNA155) as a bridge, NaGdF:Yb,Er@NaYF UCNPs and AuNPs were conjugated to form NaGdF:Yb,Er@NaYF UCNPs-DNA-AuNPs nanocomplexes (UCNPs-DNA-AuNPs) probe. The energy transfer would occur when the distance between donor and acceptor gets closer. In the presence of target miRNA155, DNA-RNA heteroduplexes appeared as product, but the luminescence intensity was not changed obviously. In the existence of duplex-specific nuclease (DSN), DSN could hydrolyze the DNA strand of DNA-RNA heteroduplexes, the bridge linked NaGdF:Yb,Er@NaYF UCNPs and AuNPs was destroyed, which induced that the quenched luminescence intensity was recovered and RNA was released. The released miRNA155 could react with another UCNPs-DNA-AuNPs probe to form DNA-RNA heteroduplexes again. This cyclic reaction generates an amplification of luminescence signal for quantitative detection of miRNA155. Under the illumination of 980 nm laser, the concentration ranges from 0.1 nM to 15 nM and the detection of limits was 0.045 nM for detection of miRNA155. Moreover, the UCNPs-DNA-AuNPs probe was used in quantify miRNA155 in cell lysates with satisfactory results.
一种新型的基于上转换纳米粒子（UCNPs）和金纳米粒子（AuNPs）的发光能量转移（LET）探针，用于检测肿瘤相关 microRNA。该探针以 NaGdF:Yb,Er@NaYF 上转换纳米粒子作为能量供体，以 AuNPs 作为能量受体，利用 microRNA155（miRNA155）的双链修饰互补 DNA 序列作为桥梁，将 NaGdF:Yb,Er@NaYF UCNPs 和 AuNPs 连接起来，形成 NaGdF:Yb,Er@NaYF UCNPs-DNA-AuNPs 纳米复合物（UCNPs-DNA-AuNPs）探针。当供体和受体之间的距离变近时，能量转移会发生。在存在靶 miRNA155 的情况下，会出现 DNA-RNA 杂合体作为产物，但发光强度没有明显变化。在双链特异性核酸酶（DSN）存在的情况下，DSN 可以水解 DNA-RNA 杂合体的 DNA 链，连接 NaGdF:Yb,Er@NaYF UCNPs 和 AuNPs 的桥梁被破坏，导致猝灭发光强度恢复并释放 RNA。释放的 miRNA155 可以与另一个 UCNPs-DNA-AuNPs 探针再次反应形成 DNA-RNA 杂合体。这种循环反应产生了发光信号的放大，用于定量检测 miRNA155。在 980nm 激光的照射下，该探针对 miRNA155 的检测范围为 0.1nM 至 15nM，检测限为 0.045nM。此外，该 UCNPs-DNA-AuNPs 探针还用于细胞裂解液中 miRNA155 的定量检测，结果令人满意。
