与 N-酰基高丝氨酸内酯相关的活性污泥中群体感应细菌的候选物。
Candidates of quorum sensing bacteria in activated sludge associated with N-acyl homoserine lactones.
机构信息
Department of Environmental Design, Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa, 920-1192, Japan.
Department of Urban Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo, 113-8656, Japan; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, 113-8657, Japan.
出版信息
Chemosphere. 2019 Dec;236:124292. doi: 10.1016/j.chemosphere.2019.07.023. Epub 2019 Jul 8.
This study aimed to explore candidates of microbial groups which is associated with quorum sensing in activated sludge. Activated sludge samples were collected from three wastewater treatment plants (WWTP) to analyze N-acyl homoserine lactone (AHL) by Fourier-transform mass spectrometry (FTMS) and 16S rRNA-based microbial community. Among activated sludge samples taken at 3 WWTPs in different seasons, 2 AHL species of N-3-hydroxyoctanoyl-l-homoserine lactone and N-3-hydroxydecanoyl-l-homoserine lactone were detected in the range of ranged of 0.1 ng/L to 1.6 ng/L. The detected AHL species were not dependent on treatment systems nor seasons. From microbial community analysis, population abundance of one strain in Verrucomicrobia and two strains in Holophagaceae had high correlation with AHL concentration in activated sludge. Comamonadaceae had also moderately correlated population with AHL concentrations among quorum sensing bacteria reported previously.
本研究旨在探索与活性污泥中群体感应相关的微生物群体候选物。从三个污水处理厂（WWTP）采集活性污泥样本，通过傅里叶变换质谱（FTMS）和基于 16S rRNA 的微生物群落分析 N-酰基高丝氨酸内酯（AHL）。在不同季节从 3 个 WWTP 采集的活性污泥样本中，在 0.1ng/L 至 1.6ng/L 的范围内检测到两种 AHL 物质：N-3-羟基辛酰基-L-高丝氨酸内酯和 N-3-羟基癸酰基-L-高丝氨酸内酯。检测到的 AHL 物质不依赖于处理系统或季节。从微生物群落分析中，疣微菌门中的一个菌株和 Holophagaceae 中的两个菌株的种群丰度与活性污泥中 AHL 浓度具有高度相关性。Comamonadaceae 与先前报道的群体感应细菌的 AHL 浓度也具有中等相关性。
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