Ailenberg M, Tung P S, Pelletier M, Fritz I B
Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.
Endocrinology. 1988 Jun;122(6):2604-12. doi: 10.1210/endo-122-6-2604.
A two-chamber assembly has been employed to investigate influences of peritubular cells (PC) and extracellular matrix (Matrigel) on barrier formation by Sertoli cells (SC) in culture and on transferrin production. The kinetics of transport of [3H]inulin across a Millipore filter were essentially the same in the presence or absence of Matrigel or PC. In contrast, a SC monolayer retarded the diffusion of [3H]inulin, increasing the estimated time for 50% equilibration from about 4 h to approximately 12 h. Matrigel and PC each independently further increased the equilibration time, with the largest effects elicited by the presence of Matrigel (approximately 21 h). Data have been interpreted to indicate that these two components, especially extracellular matrix, facilitate the formation of a functional barrier by SC in the two-chamber system. PC assume a more important role than Matrigel in the modulation of transferrin secretion by SC. Transferrin concentrations were higher in the inner chamber, corresponding to those in the adluminal compartment, but transferrin masses were higher in the outer chamber under the conditions described. We report the effects of the presence and absence of Matrigel, PC, and FSH on levels of transferrin secreted by SC. Addition of FSH resulted in increased transferrin secretion by SC maintained under all conditions examined. We compare our data with those previously reported by others and attempt to provide a basis for the differences observed. We discuss the properties of the system and outline major advantages and limits of the two-chamber assembly in investigations on the polarity and properties of SC in culture.
采用双室组件研究了肾小管周细胞（PC）和细胞外基质（基质胶）对培养的支持细胞（SC）形成屏障及转铁蛋白产生的影响。在有或没有基质胶或PC的情况下，[³H]菊粉通过微孔滤膜的转运动力学基本相同。相比之下，SC单层会阻碍[³H]菊粉的扩散，使估计的50%平衡时间从约4小时增加到约12小时。基质胶和PC各自独立地进一步延长了平衡时间，其中基质胶的存在产生的影响最大（约21小时）。数据表明，这两种成分，尤其是细胞外基质，有助于在双室系统中由SC形成功能性屏障。在调节SC分泌转铁蛋白方面，PC比基质胶发挥更重要的作用。内室中的转铁蛋白浓度较高，与近腔室中的浓度相对应，但在所述条件下，外室中的转铁蛋白质量较高。我们报告了有无基质胶、PC和促卵泡激素（FSH）对SC分泌的转铁蛋白水平的影响。添加FSH导致在所有检测条件下培养的SC分泌的转铁蛋白增加。我们将我们的数据与其他人先前报道的数据进行比较，并试图为观察到的差异提供依据。我们讨论了该系统的特性，并概述了双室组件在研究培养的SC的极性和特性方面的主要优点和局限性。
