Hassan S, Skilton R A, Pelle R, Odongo D, Bishop R P, Ahmed J, Seitzer U, Bakheit M, Hassan S M, El Hussein A M
Central Veterinary Research Laboratory, Khartoum, Sudan.
Biosciences eastern and central Africa - International Livestock Research Institute Hub (BecA-ILRI Hub), Kenya.
Prev Vet Med. 2019 Aug 1;169:104697. doi: 10.1016/j.prevetmed.2019.104697. Epub 2019 May 22.
Malignant theileriosis of sheep and goats caused by Theileria lestoquardi is considered to be among the most important tick borne diseases in the Sudan. Information on the prevalence of the disease in different parts of the Sudan is limited. The purpose of this study was to estimate the prevalence of the disease in five states of the Sudan using molecular and serological assays. A total of 393 blood and serum samples from clinically asymptomatic sheep were analysed using nested reverse line blot (nRLB) and loop mediated isothermal amplification (LAMP), as well as an enzyme-linked immunosorbent assay (ELISA). The results indicated a sero-prevalence of 33.8% while RLB and LAMP assays revealed molecular prevalences of 29.5 and 22.6% respectively. The prevalence of Theileria lestoquardi varied significantly according to the geographical origin of the infected animals, whereas age and gender did not have a significant effect. RLB data indicated that T. lestoquardi usually occurred as a co-infection with the non-pathogenic Theileria ovis. Using RLB as a gold standard, a sensitivity of 68.1% and a specificity of 96.4% were recorded for LAMP and a sensitivity of 75.9% and a specificity of 83.8% for ELISA. The Kappa coefficient between nRLB and LAMP indicated a significant level of agreement (0.692), but only moderate concordance (0.572) between nRLB and ELISA. The results of the present study confirm and extend earlier findings regarding the widespread of T. lestoquardi infections in sheep in the Sudan. The data provide evidence that should enable the veterinary authorities to deploy appropriate control measures.
由莱氏泰勒虫（Theileria lestoquardi）引起的绵羊和山羊恶性泰勒虫病被认为是苏丹最重要的蜱传疾病之一。关于该疾病在苏丹不同地区的流行情况的信息有限。本研究的目的是使用分子和血清学检测方法估计苏丹五个州该疾病的流行率。对来自临床无症状绵羊的总共393份血液和血清样本进行了巢式反向线杂交（nRLB）、环介导等温扩增（LAMP）以及酶联免疫吸附测定（ELISA）分析。结果表明血清流行率为33.8%，而RLB和LAMP检测分别显示分子流行率为29.5%和22.6%。莱氏泰勒虫的流行率根据感染动物的地理来源有显著差异，而年龄和性别没有显著影响。RLB数据表明，莱氏泰勒虫通常与非致病性的绵羊泰勒虫（Theileria ovis）共同感染。以RLB作为金标准，LAMP的灵敏度为68.1%，特异性为96.4%，ELISA的灵敏度为75.9%，特异性为83.8%。nRLB与LAMP之间的Kappa系数表明一致性水平较高（0.692），但nRLB与ELISA之间只有中等程度的一致性（0.572）。本研究结果证实并扩展了关于苏丹绵羊中莱氏泰勒虫感染广泛存在的早期发现。这些数据提供了证据，应能使兽医当局采取适当的控制措施。
