Selective control of rat granulosa cell inhibin production by FSH and LH in vitro.

We studied the role of luteinising hormone (LH) and human chorionic gonadotropin (hCG) in regulation of rat granulosa cell inhibin production. Whereas pregnant mare serum gonadotropin (PMSG) or purified rat follicle-stimulating hormone (FSH) stimulated inhibin accumulation in culture media up to 6-fold in a dose-dependent manner, hCG or LH alone were without significant effect. Concomitant addition of hCG or LH to cultures containing half maximal or maximal stimulating concentrations of PMSG did, however, result in a dose-dependent inhibition of PMSG/FSH-induced inhibin production. However, in the 2-step culture system a biphasic effect of hCG treatment on FSH-primed granulosa cell inhibin and progesterone production was evident. hCG was only inhibitory when the cells in the 2-step system were primed with PMSG. These data indicate that granulosa cell inhibin production is under direct control of both FSH and LH, and provide a possible explanation for alterations in inhibin activity around the time of ovulation in vivo.