Shibahara Hidenori, Ishiguro Ai, Shirasuna Koumei, Kuwayama Takehito, Iwata Hisataka
Department of Animal Reproduction Tokyo University of Agriculture Atsugi Japan.
Reprod Med Biol. 2019 Apr 10;18(3):256-262. doi: 10.1002/rmb2.12269. eCollection 2019 Jul.
This study aimed to examine the relationship between granulosa cells (GCs), number of follicles, and the ability of follicular fluid to support in vitro growth of oocytes.
The culture medium was supplemented with follicular fluid (FF) collected from GC-rich ovaries and GC-poor ovaries, and its effect on in vitro growth and quality of oocytes derived from early antral follicles (EAFs) was assessed.
GC-rich FF treatment enhanced oocyte growth and augmented changes in the chromatin configuration and lipid content of oocytes when compared to oocytes treated with GC-poor FF. Moreover, oocytes treated with GC-rich FF had a higher ability to progress to the blastocyst stage, than oocytes derived from large antral follicles (3-5 mm in diameter). In addition, supplementation of the culture medium with either GC-rich or GC-poor FF enhanced histone acetylation in oocytes grown in vitro.
GC-rich FF contains key factors that support in vitro oocyte growth; hence, oocytes grown in GC-rich FF medium had high developmental competence, which was comparative to the oocytes grown in vivo.
本研究旨在探讨颗粒细胞（GCs）、卵泡数量与卵泡液支持卵母细胞体外生长能力之间的关系。
向培养基中添加从富含GCs的卵巢和缺乏GCs的卵巢收集的卵泡液（FF），并评估其对来自早期窦卵泡（EAFs）的卵母细胞体外生长和质量的影响。
与用缺乏GCs的FF处理的卵母细胞相比，富含GCs的FF处理可促进卵母细胞生长，并增加卵母细胞染色质构型和脂质含量的变化。此外，用富含GCs的FF处理的卵母细胞比来自大窦卵泡（直径3 - 5毫米）的卵母细胞具有更高的发育至囊胚阶段的能力。此外，向培养基中添加富含GCs或缺乏GCs的FF均可增强体外培养的卵母细胞中的组蛋白乙酰化。
富含GCs的FF含有支持卵母细胞体外生长的关键因子；因此，在富含GCs的FF培养基中生长的卵母细胞具有较高的发育能力，这与体内生长的卵母细胞相当。
