Kung J T
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284.
J Immunol. 1988 Jun 1;140(11):3727-35.
A comparative study of the phenotype and immune functions of highly purified CD8+CD4- T cells obtained from the spleen and thymus of normal mice and from the spleen of athymic nude mice was conducted. Of seven individual normal and nude mice examined, the range of V beta 8+ cells among CD8+ T cells was a heterogeneous 4.3 to 30.5% for athymic nude mice and a much more uniform spread from 14.7 to 18.5% for normal mice. In six of the seven nude mice examined, the fraction of V beta 8+ cells was below the lower limit of the V beta 8 distribution in normal mice. However, one of the seven nude mice contained nearly twice the percentage of normal V beta 8+ cells. A reduction in the density of V beta 8 as well as CD3 Ag expression was also observed in athymic CD8+CD4- cells although an Ly-6-linked Ag, B4B2 displayed a highly increased expression. Considering the battery of Ag analyzed in entirety, athymic CD8+CD4- T cells were clearly distinct from their "counterpart" CD8+CD4- T cells isolated from either thymus or spleen of normal (euthymic) mice. Anti-CD3-mediated triggering of the TCR:CD3 complex caused extensive clonal proliferation in cultures to which single responding CD8+ T cells had been deposited. Under identical conditions, however, anti-CD3 caused little, if any clonal expansion in CD8+ cells from athymic nude mice. Highly purified athymic CD8+CD4- cells produced readily detectable IL-2R expression and IL-2 synthesis and secretion upon stimulation by anti-CD3 and by Con A. Production of IL-2 by purified athymic CD8+CD4- cells was due to CD8+CD4- cells and not due to a minor population of contaminating CD8- cells as anti-CD8 + C treatment completely abrogated the ability of athymic CD8+CD4- cells to produce IL-2. Despite IL-2 production and IL-2R expression by athymic nude CD8+CD4- T cells in response to anti-CD3 and to Con A, an impaired proliferative response followed.
对从正常小鼠的脾脏和胸腺以及无胸腺裸鼠的脾脏中获得的高度纯化的CD8 + CD4 - T细胞的表型和免疫功能进行了比较研究。在检查的7只正常小鼠和裸鼠个体中，无胸腺裸鼠的CD8 + T细胞中Vβ8 +细胞的范围为异质性的4.3%至30.5%，而正常小鼠的范围则更为均匀，为14.7%至18.5%。在检查的7只裸鼠中的6只中，Vβ8 +细胞的比例低于正常小鼠中Vβ8分布的下限。然而，7只裸鼠中的1只所含正常Vβ8 +细胞的百分比几乎是正常水平的两倍。在无胸腺的CD8 + CD4 - 细胞中也观察到Vβ8密度以及CD3抗原表达的降低，尽管一种与Ly - 6相关的抗原B4B2显示出高度增加的表达。从整体分析的一系列抗原来看，无胸腺的CD8 + CD4 - T细胞明显不同于从正常（有胸腺）小鼠的胸腺或脾脏中分离出的“对应”CD8 + CD4 - T细胞。抗CD3介导的TCR:CD3复合物触发在接种了单个反应性CD8 + T细胞的培养物中引起广泛的克隆增殖。然而，在相同条件下，抗CD3在来自无胸腺裸鼠的CD8 +细胞中几乎没有引起克隆扩增（如果有也很少）。高度纯化的无胸腺CD8 + CD4 - 细胞在抗CD3和刀豆蛋白A刺激后产生易于检测到的IL - 2R表达以及IL - 2的合成和分泌。纯化的无胸腺CD8 + CD4 - 细胞产生IL - 2是由于CD8 + CD4 - 细胞，而不是由于少量污染的CD8 - 细胞群体，因为抗CD8 + C处理完全消除了无胸腺CD8 + CD4 - 细胞产生IL - 2的能力。尽管无胸腺裸鼠的CD8 + CD4 - T细胞在响应抗CD3和刀豆蛋白A时产生IL - 2并表达IL - 2R，但随后的增殖反应受损。
