Bandeira A, Itohara S, Bonneville M, Burlen-Defranoux O, Mota-Santos T, Coutinho A, Tonegawa S
Unite d'immunobiologie, Institut Pasteur, Paris, France.
Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):43-7. doi: 10.1073/pnas.88.1.43.
The kinetics of postnatal intestinal colonization by T cells carrying gamma delta and alpha beta T-cell antigen receptors were studied in nude and normal mice by flow cytometry and immunohistology. Furthermore, gamma delta and alpha beta T-cell development was analyzed in lethally irradiated mice that were reconstituted by fetal liver precursors with or without a thymus. Our results establish that a major subpopulation of gamma delta intestinal intraepithelial lymphocytes is produced from uncommitted precursors at extrathymic sites. This work further shows that a small pool of T cells carrying alpha beta T-cell receptors can also differentiate extrathymically from CD3- fetal liver precursors but with rates of production and peripheral expansion much reduced as compared with those observed in thymus-bearing animals.
通过流式细胞术和免疫组织学方法，对裸鼠和正常小鼠中携带γδ和αβ T细胞抗原受体的T细胞在出生后肠道定植的动力学进行了研究。此外，还对接受或未接受胸腺的胎肝前体重建的致死性照射小鼠中的γδ和αβ T细胞发育进行了分析。我们的结果表明，γδ肠道上皮内淋巴细胞的一个主要亚群是由胸腺外部位未定向的前体产生的。这项工作进一步表明，一小部分携带αβ T细胞受体的T细胞也可以从CD3-胎肝前体在胸腺外分化，但与有胸腺动物相比，其产生率和外周扩增率大大降低。
