Department of Medicine, Cardenal Herrera-CEU University, CEU Universities, Castellón, Spain.
Parasitology Reference and Research Laboratory, National Centre for Microbiology, Health Institute Carlos III, Majadahonda, Spain.
Vector Borne Zoonotic Dis. 2019 Dec;19(12):915-922. doi: 10.1089/vbz.2019.2468. Epub 2019 Jul 17.
The diversity and frequency of enteric parasites in dog populations in the Castellón province (Eastern Spain) were assessed using a prospective cross-sectional epidemiological survey. A total of 263 canine fecal samples were collected between July 2014 and July 2016. Detection of intestinal parasites was conducted by routine coprological methods. In addition, identification of and spp. was carried out by direct immunofluorescence microscopy, whereas the presence of spp. was assessed by real-time PCR in a selected number of specimens. Based on conventional and/or immunofluorescence microscopy examination, 65.8% (95% confidence interval: 59.7-71.5) of the investigated dogs were found infected by at least one gastrointestinal parasite. (35.4%) and members of the family Ancylostomatidae (27.0%) were the most prevalent protozoan and helminth parasites found, respectively. Other pathogens potentially infective to humans included (8.0%), spp. (6.8%), and spp. (1.1%). Frequency of occurrence of helminthic, but not protozoan, enteroparasites was geographical origin dependent ( = 0.02), with dogs living in coastal areas presenting higher infection rates than those living in inland regions. Similarly, rural dogs were significantly more infected than urban dogs ( < 0.001). Our results revealed that zoonotic agents were common in dogs from the Castellón province. Animals from rural areas and sheltered dogs were particularly at risk of these infections.
采用前瞻性横断面流行病学调查评估了西班牙东部卡斯特利翁省（Castellón province）犬种群中的肠道寄生虫的多样性和频率。2014 年 7 月至 2016 年 7 月期间共采集了 263 份犬粪便样本。通过常规粪便检查方法检测肠道寄生虫。此外，通过直接免疫荧光显微镜检测 和 种，在选定数量的标本中通过实时 PCR 评估 的存在。基于常规和/或免疫荧光显微镜检查，发现 65.8%（95%置信区间：59.7-71.5）的受调查犬至少感染了一种胃肠道寄生虫。原虫中最常见的是 （35.4%），蠕虫中最常见的是Ancylostomatidae 科的成员（27.0%）。其他对人类具有潜在传染性的病原体包括 （8.0%）、 spp.（6.8%）和 spp.（1.1%）。蠕虫性寄生虫的发生频率（而非原虫）与地理起源有关（ = 0.02），生活在沿海地区的犬比生活在内陆地区的犬感染率更高。同样，农村犬比城市犬的感染率显著更高（ < 0.001）。我们的研究结果表明，卡斯特利翁省的犬中常见的是人畜共患病原体。来自农村地区的动物和收容所犬特别容易受到这些感染的影响。
