衰老人体骨骼肌失神经支配的分子标志物。
Molecular indicators of denervation in aging human skeletal muscle.
机构信息
Institute of Sports Medicine Copenhagen, Department of Orthopaedic Surgery M, Bispebjerg Hospital, Copenhagen, Denmark.
Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
出版信息
Muscle Nerve. 2019 Oct;60(4):453-463. doi: 10.1002/mus.26638. Epub 2019 Jul 29.
INTRODUCTION
Muscle fiber denervation increases with age, yet studies at the tissue level are sparse due to the challenging nature of establishing the relative role of regeneration and denervation.
METHODS
Muscle biopsies were obtained from the vastus lateralis of 70 healthy men (aged 72 ± 6 years; range, 65-94). Messenger RNA (mRNA) levels of acetylcholine receptors (AchR) were measured, and sections were stained for embryonic myosin, neonatal myosin (MHC ), and neural cell adhesion molecule (NCAM).
RESULTS
Embryonic myosin fibers were rare, while MHC and NCAM fibers were observed in all samples. Age (range, 65-94 years) was negatively associated with AchRγ mRNA.
DISCUSSION
Muscle from healthy older individuals expressed developmental myosins to varying degrees but more than has been previously reported for young individuals. Along with the AchR correlations, we propose that these findings support the presence of neuromuscular junction destabilization, denervation, and reinnervation in aging human skeletal muscle.
简介
肌肉纤维失神经支配随着年龄的增长而增加，但由于确立再生和失神经支配的相对作用具有挑战性，因此组织水平的研究很少。
方法
从 70 名健康男性（年龄 72±6 岁；范围，65-94）的股外侧肌获得肌肉活检。测量乙酰胆碱受体 (AchR) 的信使 RNA (mRNA) 水平，并对胚胎肌球蛋白、新生肌球蛋白 (MHC) 和神经细胞粘附分子 (NCAM) 进行染色。
结果
胚胎肌球蛋白纤维很少，而 MHC 和 NCAM 纤维在所有样本中均有观察到。年龄（范围，65-94 岁）与 AchRγ mRNA 呈负相关。
讨论
健康老年人的肌肉表现出不同程度的发育性肌球蛋白，但比以前报道的年轻人更多。结合 AchR 的相关性，我们提出这些发现支持在衰老的人类骨骼肌中存在神经肌肉接头失稳、失神经支配和再支配。
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