a College of Life Science, Henan Normal University , Xinxiang , China.
b State Key Laboratory Cultivation Base for Cell Differentiation Regulation , Xinxiang , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):2957-2966. doi: 10.1080/21691401.2019.1640710.
Researchers have been exploring the genetic mechanisms underlying the control of liver regeneration (LR). However, an integrated analysis of circRNAs expression of rat regenerating livers during the proliferation phase has not been performed yet. For this purpose, circRNAs expression profile was globally analyzed by high-throughput sequencing. It showed that 10,003 circRNAs were detected, and 164 circRNAs were differentially expressed. Subsequently, 27 circRNAs were predicted to bind to 58 candidate miRNAs and compete for miRNA-binding sites with 2195 mRNAs. By applying GO and KEGG analysis, it was predicted that these circRNAs significantly participated in tissue regeneration, regulation of cell proliferation and Ras, p53, Wnt, Jak-STAT, MAPK signalling pathways. Based on the number of the corresponding miRNAs and their role enriched and reported in cell proliferation of LR or hepatocellular carcinoma, four kinds of circRNAs (circ_03848, circ_08236, circ_13398 and circ_15013) were considered as the key circRNAs. The predicted competing endogenous RNA networks and bioinformatics analysis revealed the potential role of these circRNAs in LR, which would provide useful information for understanding the mechanism of LR.
研究人员一直在探索控制肝脏再生（LR）的遗传机制。然而，尚未对增殖阶段大鼠再生肝脏中的 circRNAs 表达进行综合分析。为此，通过高通量测序对 circRNAs 表达谱进行了全面分析。结果显示，检测到 10003 个 circRNAs，其中 164 个 circRNAs差异表达。随后，预测 27 个 circRNAs 与 58 个候选 miRNA 结合，并与 2195 个 mRNAs 竞争 miRNA 结合位点。通过 GO 和 KEGG 分析，预测这些 circRNAs 显著参与组织再生、细胞增殖和 Ras、p53、Wnt、Jak-STAT、MAPK 信号通路的调节。基于对应 miRNA 的数量及其在 LR 或肝细胞癌细胞增殖中的富集和报道作用，将 4 种 circRNAs（circ_03848、circ_08236、circ_13398 和 circ_15013）视为关键 circRNAs。预测的竞争内源 RNA 网络和生物信息学分析揭示了这些 circRNAs 在 LR 中的潜在作用，为理解 LR 机制提供了有用信息。
