Department of Ultrasonography, Second Affiliated People's Hospital of Fujian Traditional Chinese Medicine University, Fuzhou, Fujian, China.
Department of Cardiology, Second Affiliated People's Hospital of Fujian Traditional Chinese Medicine University, Fuzhou, Fujian, China.
Dis Markers. 2019 Jun 17;2019:4823156. doi: 10.1155/2019/4823156. eCollection 2019.
Apelin plays important roles in cardiovascular homeostasis. However, its effects on the mechanoenergetics of heart failure (HF) are unavailable. We attempted to investigate the effects of apelin on the left ventricular-arterial coupling (VAC) and mechanical efficiency in rats with HF. HF was induced in rats by the ligation of the left coronary artery. The ischemic HF rats were treated with apelin or saline for 12 weeks. The sham-operated animals served as the control. The left ventricular (LV) afterload and the systolic and diastolic functions, as well as the mechanoenergetic indices were estimated from the pressure-volume loops. Myocardial fibrosis by Masson's trichrome staining, myocardial apoptosis by TUNEL, and collagen content in the aorta as well as media area in the aorta and the mesenteric arteries were determined. Our data indicated that HF rats manifested an increased arterial load (Ea), a declined systolic function (reduced ejection fraction, +dP/dt, end-systolic elastance, and stroke work), an abnormal diastolic function (elevated end-diastolic pressure, , and declined -dP/dt), and decreased mechanical efficiency. Apelin treatment improved those indices. Concomitantly, increased fibrosis in the LV myocardium and the aorta and enhanced apoptosis in the LV were partially restored by apelin treatment. A declined wall-to-lumen ratio in the mesenteric arteries of the untreated HF rats was further reduced in the apelin-treated group. We concluded that the rats with ischemic HF were characterized by deteriorated LV mechanoenergetics. Apelin improved mechanical efficiency, at least in part, due to the inhibiting cardiac fibrosis and apoptosis in the LV myocardium, reducing collagen deposition in the aorta and dilating the resistant artery.
Apelin 在心血管稳态中发挥重要作用。然而，其对心力衰竭（HF）的机械能量学的影响尚不清楚。我们试图研究 Apelin 对 HF 大鼠左心室-动脉偶联（VAC）和机械效率的影响。通过结扎左冠状动脉诱导 HF。缺血性 HF 大鼠用 Apelin 或生理盐水治疗 12 周。假手术动物作为对照。从压力-容积环估计左心室（LV）后负荷和收缩和舒张功能以及机械能量学指标。通过 Masson 三色染色测定心肌纤维化、TUNEL 测定心肌凋亡以及主动脉胶原含量和主动脉及肠系膜动脉中膜面积。我们的数据表明，HF 大鼠表现出动脉负荷增加（Ea）、收缩功能下降（射血分数降低、+dP/dt、收缩末期弹性和每搏功降低）、舒张功能异常（舒张末期压力升高、和 -dP/dt 降低）和机械效率降低。Apelin 治疗改善了这些指标。同时，Apelin 治疗部分恢复了 LV 心肌和主动脉中增加的纤维化以及 LV 中的凋亡增加。未治疗的 HF 大鼠肠系膜动脉的壁腔比进一步降低，在 Apelin 治疗组中进一步降低。我们得出结论，缺血性 HF 大鼠的左心室机械能量学恶化。Apelin 通过抑制 LV 心肌中的纤维化和凋亡、减少主动脉中的胶原沉积和扩张阻力动脉，至少部分地提高了机械效率。
