蒜素对部分膀胱出口梗阻大鼠模型膀胱顺应性的影响。

Effect of Sulforaphane on Bladder Compliance in a Rat Model of Partial Bladder Outlet Obstruction.

机构信息

Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

出版信息

Oxid Med Cell Longev. 2019 Jun 18;2019:6026719. doi: 10.1155/2019/6026719. eCollection 2019.

AIMS

To investigate the effect of Nrf2 activator sulforaphane (SFN) on bladder compliance and the underlying mechanisms in a rat model of partial bladder outlet obstruction (BOO).

METHODS

Male 8-week-old Sprague-Dawley rats were divided into three groups. BOO rats were given daily 0.5 mg/kg sulforaphane (BOO+SFN) or vehicle (BOO) intraperitoneally for 4 weeks, while sham-operated rats were treated with vehicle (Sham). Bladder compliance, histological alteration, and collagen deposition were evaluated. The expression levels of collagen I, collagen III, MMP-1, and TIMP-1 were measured by immunohistochemistry and western blotting.

RESULTS

BOO led to a significant decrease in bladder compliance. The change was partially restored by SFN treatment. The expression of MMP-1 was significantly decreased accompanying with increased TIMP-1 expression in BOO rats compared with that in Sham rats, which was ameliorated by SFN treatment. Moreover, the increased collagen I/collagen III ratio in the BOO group was reversed by SFN treatment.

CONCLUSIONS

Sulforaphane suppressed collagen deposition by regulating the MMP-1 and TIMP-1 expression and decreasing the collagen I/III expression ratio in BOO rats and improved bladder compliance.

目的

研究 Nrf2 激活剂萝卜硫素（SFN）对部分膀胱出口梗阻（BOO）大鼠模型膀胱顺应性的影响及其机制。

方法

8 周龄雄性 Sprague-Dawley 大鼠分为 3 组。BOO 大鼠每天腹腔内给予 0.5mg/kg 萝卜硫素（BOO+SFN）或载体（BOO）4 周，假手术组大鼠给予载体（Sham）处理。评估膀胱顺应性、组织学改变和胶原沉积。通过免疫组织化学和 Western blot 检测胶原 I、胶原 III、MMP-1 和 TIMP-1 的表达水平。