Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Oxid Med Cell Longev. 2019 Jun 18;2019:6026719. doi: 10.1155/2019/6026719. eCollection 2019.
To investigate the effect of Nrf2 activator sulforaphane (SFN) on bladder compliance and the underlying mechanisms in a rat model of partial bladder outlet obstruction (BOO).
Male 8-week-old Sprague-Dawley rats were divided into three groups. BOO rats were given daily 0.5 mg/kg sulforaphane (BOO+SFN) or vehicle (BOO) intraperitoneally for 4 weeks, while sham-operated rats were treated with vehicle (Sham). Bladder compliance, histological alteration, and collagen deposition were evaluated. The expression levels of collagen I, collagen III, MMP-1, and TIMP-1 were measured by immunohistochemistry and western blotting.
BOO led to a significant decrease in bladder compliance. The change was partially restored by SFN treatment. The expression of MMP-1 was significantly decreased accompanying with increased TIMP-1 expression in BOO rats compared with that in Sham rats, which was ameliorated by SFN treatment. Moreover, the increased collagen I/collagen III ratio in the BOO group was reversed by SFN treatment.
Sulforaphane suppressed collagen deposition by regulating the MMP-1 and TIMP-1 expression and decreasing the collagen I/III expression ratio in BOO rats and improved bladder compliance.
研究 Nrf2 激活剂萝卜硫素（SFN）对部分膀胱出口梗阻（BOO）大鼠模型膀胱顺应性的影响及其机制。
8 周龄雄性 Sprague-Dawley 大鼠分为 3 组。BOO 大鼠每天腹腔内给予 0.5mg/kg 萝卜硫素（BOO+SFN）或载体（BOO）4 周，假手术组大鼠给予载体（Sham）处理。评估膀胱顺应性、组织学改变和胶原沉积。通过免疫组织化学和 Western blot 检测胶原 I、胶原 III、MMP-1 和 TIMP-1 的表达水平。
BOO 导致膀胱顺应性显著下降。SFN 治疗部分恢复了这种变化。与 Sham 组相比，BOO 组 MMP-1 表达显著降低，同时 TIMP-1 表达增加，SFN 治疗可改善这种情况。此外，SFN 治疗可逆转 BOO 组中胶原 I/III 比值的增加。
萝卜硫素通过调节 MMP-1 和 TIMP-1 的表达，降低 BOO 大鼠胶原 I/III 的表达比值，抑制胶原沉积，从而改善膀胱顺应性。
