Song Meiyi, Sun Mengxue, Xia Lu, Chen Wei, Yang Changqing
Division of Gastroenterology and Hepatology, Digestive Disease Institute, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
Emergency Department, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China.
Ann Transl Med. 2019 Jun;7(11):236. doi: 10.21037/atm.2019.04.61.
Pancreatic cancer is a common cancer with a poor prognosis and an increasing morbidity. miR-19b-3p has been implicated in some cancers, however, its role in pancreatic cancer is unclear.
Human pancreatic cancer cell line Capan-2 cells were transfected with miR-19b-3p mimic and inhibitor. Cell proliferation was measured by 5-Ethynyl-2'-deoxyuridine (EdU) staining assays. Cell cycle of Capan-2 cells was examined by flow cytometry. The expression of phosphatase and tension homolog (PTEN) was determined by real-time quantitative polymerase chain reaction (PCR) and western blotting analysis. Functional rescue experiments were performed through PTEN overexpression and miR-19b-3p mimic by using EdU staining assays.
miR-19b-3p mimic significantly increased miR-19b-3p while miR-19b-3p inhibitor decreased that. EdU staining showed that miR-19b-3p overexpression promoted Capan-2 cells proliferation while miR-19b-3p inhibition decreased that. Flow cytometry analysis of cell cycle indicated that miR-19b-3p overexpression increased the percentage of Capan-2 cells in S phase while miR-19b-3p inhibition decreased that. was confirmed to be a target gene of miR-19b-3p and overexpression eliminated the pro-proliferation effects of miR-19b-3p in Capan-2 cells.
Our study demonstrates that miR-19b-3p promotes Capan-2 cells proliferation by targeting .
胰腺癌是一种常见癌症，预后较差且发病率不断上升。miR-19b-3p已被证明与某些癌症有关，然而，其在胰腺癌中的作用尚不清楚。
用miR-19b-3p模拟物和抑制剂转染人胰腺癌细胞系Capan-2细胞。通过5-乙炔基-2'-脱氧尿苷（EdU）染色试验检测细胞增殖。通过流式细胞术检测Capan-2细胞的细胞周期。通过实时定量聚合酶链反应（PCR）和蛋白质印迹分析测定磷酸酶和张力蛋白同源物（PTEN）的表达。通过使用EdU染色试验，通过PTEN过表达和miR-19b-3p模拟物进行功能挽救实验。
miR-19b-3p模拟物显著增加miR-19b-3p水平，而miR-19b-3p抑制剂则降低其水平。EdU染色显示，miR-19b-3p过表达促进Capan-2细胞增殖，而miR-19b-3p抑制则降低其增殖。细胞周期的流式细胞术分析表明，miR-19b-3p过表达增加了Capan-2细胞在S期的百分比，而miR-19b-3p抑制则降低了该百分比。PTEN被证实是miR-19b-3p的靶基因，PTEN过表达消除了miR-19b-3p在Capan-2细胞中的促增殖作用。
我们的研究表明，miR-19b-3p通过靶向PTEN促进Capan-2细胞增殖。
