Matsushita Tomonao
Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Methods Mol Biol. 2019;2026:143-148. doi: 10.1007/978-1-4939-9612-4_11.
Recently, phytochrome has been shown to regulate not only transcription but also alternative splicing at a similar genomic scale in Arabidopsis. Here I describe the protocols for detecting phytochrome-mediated light-responsive alterations in alternative splicing in Arabidopsis, using RT-PCR technique followed by quantification with the Bioanalyzer.
最近，在拟南芥中已表明，光敏色素不仅在类似的基因组规模上调节转录，还调节可变剪接。在此，我描述了使用RT-PCR技术，随后用生物分析仪进行定量，来检测拟南芥中光敏色素介导的可变剪接中光响应变化的实验方案。
