Inoue Keisuke, Nishihama Ryuichi, Kohchi Takayuki
Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
Methods Mol Biol. 2019;2026:215-223. doi: 10.1007/978-1-4939-9612-4_18.
Bryophytes, which comprise liverworts, mosses, and hornworts, are one of the earliest diverging lineages of extant land plants and a key plant group for understanding evolutionary aspects of land plant adaptation. Marchantia polymorpha, a liverwort, has recently been established as a model plant species having molecular genetic tractability. In M. polymorpha, phytochrome is encoded by a single-copy gene, MpPHY, with Mpphy regulating various physiological responses through PHYTOCHROME INTERACTING FACTOR (PIF)-mediated transcriptional regulation. The phytochrome signaling system of M. polymorpha, with its single Mpphy and single PIF (MpPIF), is relatively simple compared with other model plants carrying multiple phytochromes and PIFs. Consequently, investigation of phytochrome signaling using M. polymorpha may provide novel insights into fundamental mechanisms and roles of phytochrome during the course of land plant evolution. This chapter provides a number of basic procedures, along with some tips, for designing and performing experiments with M. polymorpha to study phytochrome signaling.
苔藓植物包括叶苔、藓类和角苔，是现存陆地植物中最早分化的谱系之一，也是理解陆地植物适应性进化方面的关键植物类群。叶苔多歧灯藓最近已被确立为一种具有分子遗传易处理性的模式植物物种。在多歧灯藓中，光敏色素由单拷贝基因MpPHY编码，Mpphy通过光敏色素互作因子（PIF）介导的转录调控来调节各种生理反应。与携带多种光敏色素和PIF的其他模式植物相比，多歧灯藓的光敏色素信号系统具有单一的Mpphy和单一的PIF（MpPIF），相对简单。因此，利用多歧灯藓研究光敏色素信号可能会为陆地植物进化过程中光敏色素的基本机制和作用提供新的见解。本章提供了一些基本步骤以及一些提示，用于设计和进行多歧灯藓实验以研究光敏色素信号。
