Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden.
Department of Oncology and Pathology, Karolinska Institute, Stockholm, Sweden.
Endocr Pathol. 2019 Sep;30(3):173-179. doi: 10.1007/s12022-019-09584-3.
Neuroblastoma (NB), the most common extracranial cancer in childhood, exhibits neuroendocrine (NE) differentiation. Two well-established NE markers, chromogranin A (CgA) and synaptophysin (syn), are used in the histopathological diagnostics. Our aims were to explore if the NE markers synaptic vesicle protein 2 (SV2) and vesicular monoamine transporter 1 (VMAT1) and 2 (VMAT2) also are expressed in human NB and if so, evaluate their usefulness in NB histopathological diagnostics. Tumor specimens from 21 NB patients, before and/or after chemotherapy, were immunostained for CgA, syn, SV2, VMAT1, and VMAT2. Clinical data was extracted from patients' records. SV2 was highly expressed in NB, as was CgA while syn was less frequently expressed compared to the other two. Both VMATs were expressed in several NB, VMAT2 in more cases than VMAT1 and its expression was similar to syn. Chemotherapy did not affect the immunoreactivity in an obvious way. SV2 was highly expressed in NB and can thus be useful marker in NB diagnostics. VMAT1 and VMAT2 were also expressed in NB but similar to syn less reliable as tumor markers.
神经母细胞瘤（NB）是儿童期最常见的颅外癌症，具有神经内分泌（NE）分化。两种成熟的 NE 标志物，嗜铬粒蛋白 A（CgA）和突触素（syn），用于组织病理学诊断。我们的目的是探讨神经递质囊泡蛋白 2（SV2）和囊泡单胺转运体 1（VMAT1）和 2（VMAT2）是否也在人类 NB 中表达，如果是，评估它们在 NB 组织病理学诊断中的有用性。对 21 例 NB 患者的肿瘤标本进行 CgA、syn、SV2、VMAT1 和 VMAT2 的免疫组化染色。从患者记录中提取临床数据。SV2 在 NB 中高度表达，CgA 也是如此，而 syn 的表达则比其他两种标志物低。两种 VMATs 在一些 NB 中表达，VMAT2 的表达比 VMAT1 更常见，其表达与 syn 相似。化疗并没有明显影响免疫反应。SV2 在 NB 中高度表达，因此可以作为 NB 诊断的有用标志物。VMAT1 和 VMAT2 也在 NB 中表达，但与 syn 相似，作为肿瘤标志物不太可靠。
