CAS Key Laboratory of Soft Matter Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), Department of Chemistry , University of Science and Technology of China , Hefei , Anhui 230026 , P. R. China.
Anal Chem. 2019 Aug 20;91(16):10614-10621. doi: 10.1021/acs.analchem.9b01774. Epub 2019 Jul 30.
Chemiluminescent (CL) reagent -(4-aminobutyl)--ethylisoluminol (ABEI)-functionalized CaCO microspheres with a dual catalytic system consisting of Co and horseradish peroxidase (HRP) (HRP/ABEI/Co-CaCO MPs) were synthesized via a facile method for the first time. Co was linked onto HRP/ABEI/Co-CaCO MPs through both coordination and electrostatic interactions, while ABEI and HRP were encapsulated into HRP/ABEI/Co-CaCO MPs via electrostatic interaction and adsorption. The as-prepared HRP/ABEI/Co-CaCO MPs possessed a spherical shape with a mean size of 1.3 ± 0.3 μm and porous structure. They exhibited highly intensive "glow-type" CL emission, which was visible by naked eyes in a dark room for 10 h. The HRP/ABEI/Co-CaCO with dual catalytic system produced more intensive "glow-type" CL emission in the first 3 h as compared with ABEI/Co-CaCO and HRP/ABEI/CaCO, both of which merely possessed a single catalyst in their systems. Moreover, the CL intensity of HRP/ABEI/Co-CaCO MPs during the first hour outperforms all the previously reported long-lasting chemiluminescence-functionalized micro/nanomaterials (CF-M/NMs), such as the firefly-mimicking intensive and long-lasting ABEI/Co/chitosan hydrogels. The outstanding CL performance of HRP/ABEI/Co-CaCO MPs is attributed to high loading of CL reagents and catalysts in CaCO MPs and high catalytic efficiency and good stability of the dual catalytic system consisting of Co complex and HRP. The HRP/ABEI/Co-CaCO MPs are neutral materials with highly intensive and persistent emission as well as good biocompatibility, rendering them excellent biomaterials for various bioassays. We envisage that this excellent material holds great promise in vast application fields, such as bioassays, bioimaging, environmental monitoring, and life science etc.
化学发光（CL）试剂-（4-氨基丁基）-乙基异鲁米诺（ABEI）功能化碳酸钙微球，具有由钴和辣根过氧化物酶（HRP）组成的双催化体系（HRP/ABEI/Co-CaCO MPs），首次通过简便的方法合成。Co 通过配位和静电相互作用与 HRP/ABEI/Co-CaCO MPs 相连，而 ABEI 和 HRP 通过静电相互作用和吸附包封在 HRP/ABEI/Co-CaCO MPs 中。所制备的 HRP/ABEI/Co-CaCO MPs 具有球形形状，平均粒径为 1.3±0.3μm，具有多孔结构。它们表现出高强度的“发光型”CL 发射，在黑暗中 10 小时内肉眼可见。与仅在其系统中具有单一催化剂的 ABEI/Co-CaCO 和 HRP/ABEI/CaCO 相比，具有双催化体系的 HRP/ABEI/Co-CaCO 产生更强烈的“发光型”CL 发射，在头 3 小时内。此外，HRP/ABEI/Co-CaCO MPs 在第一小时的 CL 强度超过了所有先前报道的长效化学发光功能化微/纳米材料（CF-M/NMs），例如萤火虫模拟的高强度和长效 ABEI/Co/壳聚糖水凝胶。HRP/ABEI/Co-CaCO MPs 的出色 CL 性能归因于 CaCO MPs 中 CL 试剂和催化剂的高负载、钴配合物和 HRP 组成的双催化体系的高催化效率和良好稳定性。HRP/ABEI/Co-CaCO MPs 是中性材料，具有高强度和持久的发射以及良好的生物相容性，使其成为各种生物测定的优秀生物材料。我们预计这种优异的材料在生物测定、生物成像、环境监测和生命科学等广泛的应用领域具有广阔的应用前景。
