金雀异黄素通过下调β-连环蛋白和逆转 EMT 抑制口腔鳞状细胞癌的侵袭和增殖。
Casticin inhibits invasion and proliferation via downregulation of β-catenin and reversion of EMT in oral squamous cell carcinoma.
机构信息
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Chinese Academy of Medical Sciences Research Unit of Oral Carcinogenesis and Management, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Department of Periodontology, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
出版信息
J Oral Pathol Med. 2019 Nov;48(10):897-905. doi: 10.1111/jop.12930. Epub 2019 Aug 8.
BACKGROUND
Casticin expresses multiple anti-cancer activities, whereas the effect of casticin on oral squamous cell carcinoma (OSCC) is still unclear. β-catenin signaling plays a crucial role in the epithelial-mesenchymal transition which is closely related to tumorigenesis. Herein, we aimed to study the functions of casticin on invasion and migration of OSCC, and clarify whether the effect of casticin on OSCC has a relationship with β-catenin signaling.
METHODS
Human OSCC cell lines UM1 and HSC-3 were treated with different concentrations of casticin. The cell viability was evaluated by MTT and soft agar colony formation. Transwell assay and wound-healing assay were performed to measure the ability of cell invasion and migration. The protein expression was assessed by Western blotting.
RESULTS
Casticin displayed inhibitory activities of cell viability, invasion, and migration on OSCC cell lines. Meanwhile, casticin could reverse EMT process and inhibit the expression of β-catenin in OSCC. Knock-down or overexpression of β-catenin could alter the effect of casticin on OSCC.
CONCLUSIONS
Casticin impaired invasion and migration of OSCC by inhibition of β-catenin and reversal of EMT and could be a potential anti-cancer bioactive agent.
背景
金雀异黄素表达多种抗癌活性，但其对口腔鳞状细胞癌（OSCC）的作用尚不清楚。β-连环蛋白信号在上皮-间充质转化中起着至关重要的作用，而上皮-间充质转化与肿瘤发生密切相关。在此，我们旨在研究金雀异黄素对 OSCC 侵袭和迁移的作用，并阐明金雀异黄素对 OSCC 的作用是否与β-连环蛋白信号有关。
方法
用人 OSCC 细胞系 UM1 和 HSC-3 用不同浓度的金雀异黄素处理。通过 MTT 和软琼脂集落形成实验评估细胞活力。通过 Transwell 测定和划痕愈合试验测定细胞侵袭和迁移能力。通过 Western 印迹评估蛋白表达。
结果
金雀异黄素对 OSCC 细胞系的细胞活力、侵袭和迁移均表现出抑制活性。同时，金雀异黄素可逆转 EMT 过程并抑制 OSCC 中β-连环蛋白的表达。β-连环蛋白的敲低或过表达可改变金雀异黄素对 OSCC 的作用。
结论
金雀异黄素通过抑制β-连环蛋白和逆转 EMT 来损害 OSCC 的侵袭和迁移，可能是一种潜在的抗癌生物活性物质。
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