Department of Biosystems , MeBioS, KU Leuven , Kasteelpark Arenberg 30 , 3001 Leuven , Belgium.
Department of Biosystems, Biosystems Technology Cluster , KU Leuven Campus Geel , Kleinhoefstraat 4 , 2440 Geel , Belgium.
Anal Chem. 2019 Aug 6;91(15):10040-10048. doi: 10.1021/acs.analchem.9b01913. Epub 2019 Jul 18.
A particle size distribution (PSD) estimation method based on light-scattering properties was validated on experimental visible/near-infrared scattering spectra of polystyrene suspensions, with a nominal particle size ranging from 0.1 to 12 μm in diameter. On the basis of μ and spectra extracted from double integrating sphere measurements, good PSD estimates were obtained for particles ≥1 μm. The particle volume fraction estimates in the case of μ were close to the target concentrations, although influenced by small baseline fluctuations on the spectra. For submicrometer particles, on the other hand, the non-oscillating μ spectra lack discriminating power, resulting in erroneous PSD estimates. The reduced scattering coefficient spectra (μ') were found less useful for particle size estimation as they lack a characteristic shape, causing an over- or underestimation of the distribution width. In summary, the estimation routine proved to deliver PSD estimates in line with the reference measurements for micrometer-sized or larger particles based on their μ and scattering spectra. Additional validation on more polydisperse samples forms the next step before going to bimodal PSD estimates.
一种基于光散射特性的粒径分布（PSD）估计方法在聚苯乙烯悬浮液的实验可见/近红外散射光谱上得到了验证，其标称粒径范围为 0.1 至 12 μm。基于双积分球测量提取的 μ 和 光谱，对于粒径≥1 μm 的颗粒，得到了良好的 PSD 估计。在 μ 的情况下，颗粒体积分数的估计值接近目标浓度，尽管受到光谱上小的基线波动的影响。另一方面，对于亚微米颗粒，非振荡的 μ 光谱缺乏辨别力，导致 PSD 估计错误。由于缺少特征形状，减小的散射系数光谱（μ'）对于粒径估计的用处不大，导致分布宽度的高估或低估。总之，对于基于 μ 和 散射光谱的微米级或更大粒径的颗粒，估计程序被证明能够提供与参考测量值一致的 PSD 估计值。在进行双峰 PSD 估计之前，下一步是对更多多分散性样品进行额外验证。
