大鼠离体回肠钙和无机磷酸盐分泌。尿毒症及1,25(OH)₂D₃抑制作用的影响。

Calcium and inorganic phosphate secretion of rat ileum in vitro. Influence of uremia and 1,25 (OH)2D3 inhibition.

作者信息

Peters J, Binswanger U

机构信息

Dept. of Internal Medicine, University Hospital, Zürich, Switzerland.

出版信息

Res Exp Med (Berl). 1988;188(2):139-49. doi: 10.1007/BF01852270.

DOI:10.1007/BF01852270

PMID:3131857

Abstract

The unidirectional ileal transport of calcium (Ca) and inorganic phosphorus (Pi) of rats was measured in vitro with the modified Ussing technique. Animal preparation included 5/6 nephrectomy and EHDP treatment. They were compared to controls as well as to 1,25 (OH)2D3 supplemented rats. The results show that the ileum is a secretory organ for Ca and Pi, the serosa to mucosa transport (Jsm) exceeds the mucosa to serosa transport (Jms). Ca and Pi transport in sm direction is 1,25 (OH)2D3 independent but exhibits a mutual strong correlation. Our observations together with published data are in favor of mainly paracellular, non electrogenic sm transport of both ions. However, the factor controlling sm transport of Ca and Pi remains unidentified. The mucosa to serosa transport (Jms) in the ileum is low for both ions. Ca ms is stimulated by 1,25 (OH)2D3, Pi ms is unchanged.

摘要

采用改良的尤斯灌流技术，在体外测定大鼠回肠对钙（Ca）和无机磷（Pi）的单向转运。动物准备包括5/6肾切除和依替膦酸二钠治疗。将它们与对照组以及补充1,25（OH）2D3的大鼠进行比较。结果表明，回肠是Ca和Pi的分泌器官，浆膜到黏膜的转运（Jsm）超过黏膜到浆膜的转运（Jms）。Ca和Pi在sm方向的转运不依赖1,25（OH）2D3，但两者之间呈现出很强的相关性。我们的观察结果与已发表的数据一致，支持这两种离子主要通过细胞旁、非电生性的sm转运。然而，控制Ca和Pi的sm转运的因素仍未明确。回肠中两种离子的黏膜到浆膜转运（Jms）都很低。Ca的ms转运受1,25（OH）2D3刺激，Pi的ms转运不变。