Division of Oral and Maxillofacial Surgery, Department of Oral Medicine and Surgery, Tohoku University Graduate School of Dentistry, Sendai, Japan.
Division of Oral Pathology, Department of Oral Medicine and Surgery, Tohoku University Graduate School of Dentistry, Sendai, Japan.
J Oral Pathol Med. 2019 Nov;48(10):888-896. doi: 10.1111/jop.12925. Epub 2019 Aug 16.
Carcinogenesis occurs when the cell cycle is compromised. Chromatin licensing and DNA replication factor 1, geminin, and γ-H2A histone family member X are expressed in cells in G phase, S/G /M phases, and apoptosis, respectively, and these three markers may be useful for histological evaluation of malignant lesions. Here, we aimed to identify cell cycle phases and apoptosis using immunohistochemistry in oral epithelial precursor lesions and oral squamous cell carcinoma.
Chromatin licensing and DNA replication factor 1, geminin, and γ-H2A histone family member X expression levels were immunohistochemically examined in tissue specimens from 55 patients with oral epithelial precursor lesions and 50 patients with oral squamous cell carcinoma. Associations of clinicopathological variables with marker expression were assessed.
Chromatin licensing and DNA replication factor 1 was expressed in the prickle cell layer of oral epithelial precursor lesions and many carcinoma cells of oral squamous cell carcinoma. Geminin reactivity was widely distributed in high-grade dysplasia and oral squamous cell carcinoma rather than low-grade or no dysplastic cases. γ-H2A histone family member X was expressed in the superficial layer of oral epithelial precursor lesions and scattered carcinoma cells of oral squamous cell carcinoma. In oral squamous cell carcinoma, lower geminin expression was observed in recurrent cases. Geminin and γ-H2A histone family member X were associated with the degree of differentiation and mode of invasion.
Chromatin licensing and DNA replication factor 1, geminin, and γ-H2A histone family member X expression levels were correlated with oral carcinogenesis; these markers were associated with clinicopathological behaviors in oral squamous cell carcinoma.
细胞周期受到干扰时会发生癌变。染色质许可和 DNA 复制因子 1、增殖细胞核抗原和 γ-H2A 组蛋白家族成员 X 分别在 G 期、S/G2/M 期和细胞凋亡的细胞中表达，这三个标志物可能有助于恶性病变的组织学评估。本研究旨在通过免疫组织化学方法在口腔上皮前体病变和口腔鳞状细胞癌中鉴定细胞周期和细胞凋亡。
对 55 例口腔上皮前体病变和 50 例口腔鳞状细胞癌患者的组织标本进行染色质许可和 DNA 复制因子 1、增殖细胞核抗原和 γ-H2A 组蛋白家族成员 X 的免疫组织化学检测，并评估临床病理变量与标志物表达的相关性。
染色质许可和 DNA 复制因子 1 在口腔上皮前体病变的棘细胞层和口腔鳞状细胞癌的许多癌细胞中表达。增殖细胞核抗原在高级别发育不良和口腔鳞状细胞癌中广泛分布，而在低级别或无发育不良的病例中则分布较少。γ-H2A 组蛋白家族成员 X 在口腔上皮前体病变的浅层和口腔鳞状细胞癌的散在癌细胞中表达。在口腔鳞状细胞癌中，复发病例的增殖细胞核抗原表达较低。增殖细胞核抗原和 γ-H2A 组蛋白家族成员 X 与分化程度和浸润模式相关。
染色质许可和 DNA 复制因子 1、增殖细胞核抗原和 γ-H2A 组蛋白家族成员 X 的表达水平与口腔癌变有关；这些标志物与口腔鳞状细胞癌的临床病理行为相关。
