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用双液相浮选系统从雨生红球藻中提取天然虾青素。

Extraction of natural astaxanthin from Haematococcus pluvialis using liquid biphasic flotation system.

机构信息

Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.

Chemical Engineering Discipline, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 47500, Selangor Darul Ehsan, Malaysia.

出版信息

Bioresour Technol. 2019 Oct;290:121794. doi: 10.1016/j.biortech.2019.121794. Epub 2019 Jul 12.

DOI:10.1016/j.biortech.2019.121794
PMID:31319214
Abstract

This work aimed to study the application of liquid biphasic flotation (LBF) for the efficient and rapid recovery of astaxanthin from H. pluvialis microalgae. The performance of LBF for the extraction of astaxanthin was studied comprehensively under different operating conditions, including types and concentrations of food-grade alcohol and salt, volume ratio, addition of neutral salt, flotation period, and mass of dried H. pluvialis biomass powder. The maximum recovery, extraction efficiency and partition coefficient of astaxanthin obtained from the optimum LBF system were 95.11 ± 1.35%, 99.84 ± 0.05% and 385.16 ± 3.87, respectively. A scaled-up LBF system was also performed, demonstrating the feasibility of extracting natural astaxanthin from microalgae at a larger scale. This exploration of LBF system opens a promising avenue to the extraction of astaxanthin at lower cost and shorter processing time.

摘要

本工作旨在研究液-液双相浮选(LBF)在从雨生红球藻中高效快速回收虾青素中的应用。在不同操作条件下,包括食品级酒精和盐的类型和浓度、体积比、中性盐的添加、浮选时间和干燥雨生红球藻生物质粉末的质量,全面研究了 LBF 萃取虾青素的性能。从最佳 LBF 体系中获得的虾青素的最大回收率、提取效率和分配系数分别为 95.11±1.35%、99.84±0.05%和 385.16±3.87。还进行了放大的 LBF 系统,证明了在更大规模上从微藻中提取天然虾青素的可行性。该 LBF 系统的探索为以更低的成本和更短的加工时间提取虾青素开辟了一条有前景的途径。

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