Department of Chemistry, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, UK.
Department of Chemistry, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, UK; Department of Physics, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, UK.
J Magn Reson. 2019 Aug;305:219-231. doi: 10.1016/j.jmr.2019.07.006. Epub 2019 Jul 6.
We present a suite of two-receiver solid-state NMR experiments for backbone and side chain resonance assignment. The experiments rely on either dipolar coupling or scalar coupling for polarization transfer and are devised to acquire a H-detected 3D experiment AND a nested C-detected 2D from a shared excitation pulse. In order to compensate for the lower sensitivity of detection on C nucleus, 2D rows are signal averaged during 3D planes. The 3D dual receiver experiments do not suffer from any appreciable signal loss compared to their single receiver versions and require no extra optimization. The resulting data is higher in information content with no additional experiment time. The approach is expected to become widespread as multiple receivers become standard for new NMR spectrometers.
我们提出了一套用于骨干和侧链共振分配的双接收固体核磁共振实验。这些实验依赖于偶极耦合或标量耦合进行极化转移，并设计为从共享激发脉冲采集一个 H 检测的 3D 实验和一个嵌套的 C 检测的 2D。为了弥补 C 核检测灵敏度较低的问题，在 3D 平面期间对 2D 行进行信号平均。与单接收版本相比，3D 双接收实验没有任何明显的信号损失，也不需要额外的优化。所得数据的信息量更高，无需额外的实验时间。随着多接收成为新型 NMR 光谱仪的标准配置，这种方法有望得到广泛应用。
