BcPME37c 参与了芸薹属花粉内壁的形成。
BcPME37c is involved in pollen intine formation in Brassica campestris.
机构信息
Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China.
Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310058, China; Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou, 310058, China; Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou, 310058, China.
出版信息
Biochem Biophys Res Commun. 2019 Sep 10;517(1):63-68. doi: 10.1016/j.bbrc.2019.07.009. Epub 2019 Jul 16.
Pollen wall development is one of the key processes of pollen development. Several pectin methylesterase (PME) genes participate in pollen germination and pollen tube growth. However, the relationship between PME genes and pollen intine formation remains unclear. In this study, we investigated the expression and subcellular localization of the PME gene BcPME37c in Brassica campestris. Furthermore, morphology and cytology methods were used to examine the phenotype of the CRISPR/Cas9 system-induced BcPME37c mutant. We found that BcPME37c is predominately expressed in mature stamen and located at the cell wall. BcPME37c mutation causes the abnormal thickening of the pollen intine of B. campestris. Our study indicated that BcPME37c is required for pollen intine formation in B. campestris.
花粉壁的发育是花粉发育的关键过程之一。几个果胶甲酯酶（PME）基因参与花粉萌发和花粉管生长。然而，PME 基因与花粉内壁形成之间的关系尚不清楚。在这项研究中，我们研究了 Brassica campestris 中 PME 基因 BcPME37c 的表达和亚细胞定位。此外，还使用形态学和细胞学方法检查了 CRISPR/Cas9 系统诱导的 BcPME37c 突变体的表型。我们发现 BcPME37c 在成熟的雄蕊中表达旺盛，位于细胞壁上。BcPME37c 突变导致 Brassica campestris 花粉内壁的异常增厚。我们的研究表明，BcPME37c 是 Brassica campestris 花粉内壁形成所必需的。
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